Overview

  • Product nameAnti-Cathepsin K antibody
    See all Cathepsin K primary antibodies
  • Description
    Rabbit polyclonal to Cathepsin K
  • Specificityab19027 recognises cathepsin K.
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 300-329 of Rat Cathepsin K which includes residue 321. (Peptide available as ab41720.)

Properties

Applications

Our Abpromise guarantee covers the use of ab19027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 40, 29 kDa (predicted molecular weight: 37 kDa).Can be blocked with Cathepsin K peptide (ab41720).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionClosely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
  • Tissue specificityPredominantly expressed in osteclasts (bones).
  • Involvement in diseaseDefects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.
  • Sequence similaritiesBelongs to the peptidase C1 family.
  • Cellular localizationLysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cathepsin K antibody
    • Cathepsin O antibody
    • Cathepsin O1 antibody
    • Cathepsin O2 antibody
    • Cathepsin X antibody
    • CATK_HUMAN antibody
    • CTS02 antibody
    • Ctsk antibody
    • CTSO antibody
    • CTSO1 antibody
    • CTSO2 antibody
    • MGC23107 antibody
    • PKND antibody
    • PYCD antibody
    see all

Anti-Cathepsin K antibody images

  • ab19027 staining Cathepsin K in mouse bone tissue section, in which human MDA-MB-231 Breast Cancer cells were grown, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation and then blocking with 10% Casein for 30 minutes at 22°C was performed. The primary antibody was diluted 1/200 and incubated with sample in 10% Casein for 17 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.

    See Abreview

  • ab19027 staining Cathepsin K in the Human cell line HepaRG by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton X-100 0.1% and blocked with 1% milk for 30 minutes at 21°C. Samples were incubated with primary antibody (1ug/ml in 1% milk) for 30 minutes. An Alexa Fluor®594-conjugated Goat anti-rabbit IgG polyclonal(1/400) was used as the secondary antibody.

    See Abreview

  • ab19027 staining Cathepsin K in rat osteoclasts by Immunohistochemistry (Frozen sections). 21 day old rat bone was fixed in methanol and then blocked using 20% serum for 30 minutes at 22°C. Samples were then incubated with primary antibody at 1/50 for 3 hours at 22°C. The secondary antibody used was an anti-rabbit IgG conjugated to Cy3® (red) used at a 1/1000 dilution.
  • ab19027 staining Cathepsin K in cultured human osteoclasts by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100 PBS, blocked with 1% BSA for 1 hour at 25°C and then incubated with ab19027 at 2µg/ml for 30 minutes at 25°C. The secondary used was a FITC conjugated goat anti-rabbit polyclonal used at a 1/00 dilution.

    Red = Actin
    Blue = DAPI
    Green / Yellow = Cathepsin K

    See Abreview

References for Anti-Cathepsin K antibody (ab19027)

This product has been referenced in:
  • Manku G & Culty M Regulation of Translocator Protein 18 kDa (TSPO) Expression in Rat and Human Male Germ Cells. Int J Mol Sci 17:N/A (2016). Read more (PubMed: 27608010) »
  • Katsuyama E  et al. Interleukin-1 receptor-associated kinase-4 (IRAK4) promotes inflammatory osteolysis by activating osteoclasts and inhibiting formation of foreign body giant cells. J Biol Chem 290:716-26 (2015). IHC-P ; Mouse . Read more (PubMed: 25404736) »

See all 21 Publications for this product

Product Wall

Merci de nous avoir contactés et de nous avoir signalé le problème avec le ab19027. Les détails fournis sur le protocole utilisé vont nous permettre de prendre les dispositions nécessaires pour assurer une bonne qualité de nos produits. Comme conven...

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Application Western blot
Sample Human Cell lysate - whole cell (Vascular smooth muscle cells)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Specification Vascular smooth muscle cells
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Dec 02 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Mouse Calvarial)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 25 µg
Treatment Insulin as positive control
Specification Mouse Calvarial
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
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Submitted Aug 13 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Mouse Cell (hepatocytes)
Specification hepatocytes
Permeabilization Yes - 0.1% Triton X-100
Fixative Paraformaldehyde
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Submitted Dec 17 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step PBS 1% BSA + 5% NGS as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Sample Mouse Tissue sections (bone)
Specification bone
Permeabilization No
Fixative Formaldehyde
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Submitted Mar 24 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepaRG)
Specification HepaRG
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
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Mrs. Alina Macovei

Verified customer

Submitted Feb 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepaRG)
Loading amount 25 µg
Specification HepaRG
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Alina Macovei

Verified customer

Submitted Jun 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (Osteoclasts)
Specification Osteoclasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton x-100 PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Dec 14 2011

Thank you for your reply. As I mentioned previously, we do not give free samples of our antibodies, even for testing purposes. We issue testing discount codes for antibodies that have not been tested in a specific application or species. How the testin...

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Thank you for your reply. As mentioned in the previous emails, we do not send samples sizes of our antibodies, under our Abpomise we guarantees the antibodies to work as stated on the datasheet. If the antibodies do not work as stated then we will eith...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"