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Synthetic peptide, corresponding to a region within amino acids 300-329 of Rat Cathepsin K which includes residue 321. (Peptide available as ab41720.)
Our Abpromise guarantee covers the use of ab19027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 40, 29 kDa (predicted molecular weight: 37 kDa).Can be blocked with Cathepsin K peptide (ab41720).|
|IHC-P||Use at an assay dependent concentration.|
ab19027 staining Cathepsin K in mouse bone tissue section, in which human MDA-MB-231 Breast Cancer cells were grown, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent paraformaldehyde fixation and then blocking with 10% Casein for 30 minutes at 22°C was performed. The primary antibody was diluted 1/200 and incubated with sample in 10% Casein for 17 hours at 4°C. A Biotin conjugated goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.