Overview

  • Product name
  • Description
    Mouse monoclonal to Cathepsin K
  • Tested applications
    Suitable for: ELISA, WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Opossum
  • Immunogen

    Recombinant fragment with tag: KCRGYREIPE GNEKALKRAV ARVGPVSVAI DASLTSFQFY SKGVYYDESC NSDNLNHAVL AVGYGIQKGN KHWIIKNSWG ENWGNKGYIL MARNKNNACG IANLASFPKM, corresponding to amino acids 220-331 of Human Cathepsin K

  • Positive control
    • Cathepsin K transfected 293T cell lysate.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab66237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 10 µg/ml.

Target

Anti-Cathepsin K antibody images

  • All lanes : Anti-Cathepsin K antibody (ab66237) at 1 µg/ml

    Lane 1 : Cathepsin K transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
  • ab66237 at 10 ug/ml staining Cathepsin K in human kidney tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • ICC/IF image of ab66237 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66237, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing U20S cells stained with ab66237 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66237, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Cathepsin K antibody (ab66237)

This product has been referenced in:
  • Prieto-Potin I  et al. Characterization of multinucleated giant cells in synovium and subchondral bone in knee osteoarthritis and rheumatoid arthritis. BMC Musculoskelet Disord 16:226 (2015). IHC . Read more (PubMed: 26311062) »

See 1 Publication for this product

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I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Cathepsin K antibody tested i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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