Overview

  • Product name
  • Description
    Goat polyclonal to Cathepsin K
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Cynomolgus monkey, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    C-KTHRKQYNNKVDE

    , corresponding to internal sequence amino acids 31-43 of Human Cathepsin K (NP_000387.1).

  • Positive control
    • Human Lung and Ovary lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab77396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ELISA Use at an assay dependent dilution.

Target

Anti-Cathepsin K antibody images

  • Anti-Cathepsin K antibody (ab77396) at 1 µg/ml + Human Lung lysate (in RIPA buffer) at 35 µg

    Predicted band size : 37 kDa
    Observed band size : 37 kDa

References for Anti-Cathepsin K antibody (ab77396)

ab77396 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Tissue sections (RAW264.7 Bone marrow macrophage)
Permeabilization
Yes - 0.1 % Triton X-100 in PBS 15 min RT
Specification
RAW264.7 Bone marrow macrophage
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: +4°C
Fixative
Paraformaldehyde
Username

Tuomas Näreoja

Verified customer

Submitted Dec 22 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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