Anti-Cathepsin K antibody (ab85716)

Overview

  • Product nameAnti-Cathepsin K antibody
    See all Cathepsin K primary antibodies
  • Description
    Rabbit polyclonal to Cathepsin K
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Cathepsin K.

    (Peptide available as ab97980.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Human Bone Marrow Tissue; Human Lung Tissue; U2OS Whole Cell; MCF7 Whole Cell; MDA-MB-231 Whole Cell; MDA-MB-361 Whole Cell; Saos 2 Whole Cell.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab85716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 37 kDa).

Target

Anti-Cathepsin K antibody images

  • All lanes : Anti-Cathepsin K antibody (ab85716) at 1 µg/ml

    Lane 1 : Bone marrow (Human) Tissue Lysate
    Lane 2 : Lung (Human) Tissue Lysate
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

References for Anti-Cathepsin K antibody (ab85716)

ab85716 has not yet been referenced specifically in any publications.

Product Wall

Merci de nous avoir contactés et de nous avoir signalé le problème avec le ab19027. Les détails fournis sur le protocole utilisé vont nous permettre de prendre les dispositions nécessaires pour assurer une bonne qualité de nos produits. Comme conven...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"