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Cathepsin K Inhibitor Screening Kit (ab185439) uses the ability of active Cathepsin K to cleave the synthetic AFC based peptide substrate torelease AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin K-specific inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin K.
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Cathepsin K (CTSK, EC 184.108.40.206), a lysosomal cysteine protease, is involved in osteoclastic bone remodeling and resorption. In addition, it also degrades collagen, gelatin and elastin.
|Cathepsin K||1 vial|
|CTS K Inhibitor (FF-FMK, 1 mM)||1 x 20µl|
|CTS K Reaction Buffer||1 x 15ml|
|CTS K Reagent||1 x 100µl|
|CTS K Substrate (Ac-LR-AFC 10 mM)||1 x 0.2ml|
Our Abpromise guarantee covers the use of ab185439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab185439 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"