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Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
Abcam's Cathepsin L Activity Assay Kit is a fluorescence-based assay that utilizes the preferred cathepsin-L substrate sequence FR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-L will cleave the synthetic substrate FR-AFC to release free AFC.
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Cathepsin L plays a major role in antigen processing, tumor invasion and metastasis, bone resorption, and turnover of intracellular and secreted proteins involved in growth regulation. Although commonly recognized as a lysosomal protease, cathepsin L is also secreted. This broad-spectrum protease is potent in degrading several extracellular proteins (laminins, fibronectin, collagens I and IV, elastin, and other structural proteins of basement membranes) as well as serum proteins and cytoplasmic and nuclear proteins.
If you need additional Ac-FR-AFC substrate (10mM), it is avaible for purchasing as ab157769.
|CL Cell Lysis Buffer||WM||1 x 25ml|
|CL Inhibitor (1 mM)||Red||1 x 20µl|
|CL Reaction Buffer||NM||1 x 5ml|
|CL Substrate Ac-FR-AFC (10 mM)||Brown||1 x 200µl|
Our Abpromise guarantee covers the use of ab65306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Image obtained from Stratigopoulos G et al; JBC, 2011 Jan 21;286(3):2155-70
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"