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Rabbit polyclonal to Cathepsin L
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Cathepsin L.
(Peptide available as
This antibody gave a positive signal in both HeLa and HCT116 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 37, 25 kDa (predicted molecular weight: 37 kDa).
Important for the overall degradation of proteins in lysosomes.
Belongs to the peptidase C1 family.
Information by UniProt
cathepsin L, 1 b antibody
Cathepsin L1 antibody
Cathepsin L1 light chain antibody
Western blot - Cathepsin L antibody (ab95154)
All lanes :
Anti-Cathepsin L antibody (ab95154) at 1 µg/ml
Lane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
Additional bands at :
25 kDa (possible cleavage fragment),35 kDa (possible cleavage fragment),71 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"