Cathepsin L Human ELISA Kit (ab119509)
- Product nameCathepsin L Human ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 6.2% Inter-assay Sample n Mean SD CV% Overall 6%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum
- Assay typeSandwich (quantitative)
- Sensitivity1.7 ng/ml
- Range3.1 ng/ml - 50 ng/ml
Sample specific recovery Sample type Average % Range Serum 92 78% - 113%
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Cathepsin L Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cathepsin L concentrations in cell culture supernatant and serum. This kit is not suitable for the detection of Cathepsin L in plasma.
Cathepsin L specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Cathepsin L biotinylated detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using was buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cathepsin L captured in plate.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-human Cathepsin L monoclonal antibody 1 x 100µl Blue-Dye 1 x 400µl Green-Dye 1 x 400µl Human Cathepsin L Standard 2 vials Microplate coated with monoclonal antibody to Cathepsin L (12 x 8 wells) 1 unit Red-Dye 1 x 400µl Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 200µl TMB Substrate Solution 1 x 15ml
- Research Areas
- FunctionImportant for the overall degradation of proteins in lysosomes.
- Sequence similaritiesBelongs to the peptidase C1 family.
- Cellular localizationLysosome.
- Cathepsin L1
- Cathepsin L1 light chain
- Major excreted protein
Our Abpromise guarantee covers the use of ab119509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Cathepsin L Human ELISA Kit (ab119509)
ab119509 has not yet been referenced specifically in any publications.