Overview

  • Product nameCathepsin L Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 6.2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 6%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    1.7 ng/ml
  • Range
    3.1 ng/ml - 50 ng/ml
  • Recovery

    92 %

    Sample specific recovery
    Sample type Average % Range
    Serum 92 78% - 113%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Cathepsin L Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cathepsin L concentrations in cell culture supernatant and serum. This kit is not suitable for the detection of Cathepsin L in plasma.

    Cathepsin L specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Cathepsin L detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue product that changes to yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cathepsin L captured on the plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Cathepsin L Human ELISA Kit images

  • Representative Standard Curve using ab119509.

Protocols

References for Cathepsin L Human ELISA Kit (ab119509)

ab119509 has not yet been referenced specifically in any publications.

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Thank you for your telephone enquiry.

I can confirm as discussed on the telephone that we have the following cathepsin L ELISA kit

ab119509Cathepsin L Human ELISA Kit (with color giving dyes
http://www.abcam.com/index.html?da...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"