Overview

  • Product nameAnti-Cathepsin S antibody
    See all Cathepsin S primary antibodies
  • Description
    Rabbit polyclonal to Cathepsin S
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Cow, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Cathepsin S.

  • Positive control
    • This antibody gave a positive signal in the following Human Tissue Lysates: Lung; Spleen; Thymus

Properties

Applications

Our Abpromise guarantee covers the use of ab92780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 mg/ml. Detects a band of approximately 37,25 kDa (predicted molecular weight: 37 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

Anti-Cathepsin S antibody images

  • All lanes : Anti-Cathepsin S antibody (ab92780) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 19 kDa,25 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • ICC/IF image of ab92780 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92780, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Cathepsin S antibody (ab92780)

This product has been referenced in:
  • Wong WJ  et al. MYC degradation under low O2 tension promotes survival by evading hypoxia-induced cell death. Mol Cell Biol 33:3494-504 (2013). Read more (PubMed: 23816886) »

See 1 Publication for this product

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As we discussed on phone there are many experiments that can be performed for making sure the band seen in western blot is the actual protein of interest;

- Cross checking with multiple antibodies - It is ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"