Anti-Cathepsin V antibody [BV55-1] (ab24508)

Overview

  • Product name
    Anti-Cathepsin V antibody [BV55-1]
    See all Cathepsin V primary antibodies
  • Description
    Mouse monoclonal [BV55-1] to Cathepsin V
  • Specificity
    This antibody recognizes human Cathepsin L2 and Procathepsin L2, but not other Cathepsins.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide surrounding amino acid 21 of human cathepsin L2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, 1.0% BSA, PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    BV55-1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 20 - 30 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 37 kDa.

Target

  • Function
    Cysteine protease. May have an important role in corneal physiology.
  • Tissue specificity
    Predominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin.
  • Sequence similarities
    Belongs to the peptidase C1 family.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAT L2 antibody
    • Cathepsin L2 antibody
    • Cathepsin U antibody
    • Cathepsin V antibody
    • CathepsinL2 antibody
    • CathepsinU antibody
    • CathepsinV antibody
    • CATL 2 antibody
    • CATL2 antibody
    • CATL2_HUMAN antibody
    • CTS L2 antibody
    • CTS U antibody
    • CTS V antibody
    • CTSL 2 antibody
    • CTSL2 antibody
    • CTSU antibody
    • CTSV antibody
    • MGC125957 antibody
    • PRO305 antibody
    • UNQ268 antibody
    see all

Anti-Cathepsin V antibody [BV55-1] images

  • All lanes : Anti-Cathepsin V antibody [BV55-1] (ab24508) at 1/1000 dilution

    Lane 1 : Human hepatocyte lysate
    Lane 2 : Human liver tissue lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated goat anti-mouse IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa


    Exposure time : 28 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • IHC image of ab24508 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24508, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Cathepsin V antibody [BV55-1] (ab24508)

ab24508 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Tissue lysate - nuclear (human liver, human hepatocytes)
Specification
human liver, human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Oct 14 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A375 cells)
Loading amount
50 µg
Specification
A375 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Mar 03 2009

Thank you for filling out the technical help questionnaire, and for your patience. Your protocol looks correct but I have a few suggestions I would like you to try that should improve your signal. 1. If your lysis buffer does not contain protea...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Your complaint is the first we have received relating to this antibody. I would appreciate it if you could provide me with some further deta...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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