Recombinant
RabMAb

Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)

Overview

  • Product name
    Anti-Cathepsin V antibody [EPR10723(B)]
    See all Cathepsin V primary antibodies
  • Description
    Rabbit monoclonal [EPR10723(B)] to Cathepsin V
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Cathepsin V (UniProt ID: O60911).

  • Positive control
    • A549, HeLa, SW480, fetal kidney and Human small intestine lysates; A549 cells; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Cysteine protease. May have an important role in corneal physiology.
    • Tissue specificity
      Predominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin.
    • Sequence similarities
      Belongs to the peptidase C1 family.
    • Cellular localization
      Lysosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • CAT L2 antibody
      • Cathepsin L2 antibody
      • Cathepsin U antibody
      • Cathepsin V antibody
      • CathepsinL2 antibody
      • CathepsinU antibody
      • CathepsinV antibody
      • CATL 2 antibody
      • CATL2 antibody
      • CATL2_HUMAN antibody
      • CTS L2 antibody
      • CTS U antibody
      • CTS V antibody
      • CTSL 2 antibody
      • CTSL2 antibody
      • CTSU antibody
      • CTSV antibody
      • MGC125957 antibody
      • PRO305 antibody
      • UNQ268 antibody
      see all

    Images

    • All lanes : Anti-Cathepsin V antibody [EPR10723(B)] (ab166894) at 1/1000 dilution

      Lane 1 : A549 lysate
      Lane 2 : HeLa lysate
      Lane 3 : SW480 lysate
      Lane 4 : fetal kidney lysate
      Lane 5 : Human small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 37 kDa

    • Western blot analysis on immunoprecipitation pellet from Human small intestine lysate, using ab166894 at 1/1000 and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.
    • Immunofluorescent analysis of A549 cells labeling Cathepsin V with ab166894 at 1/250 dilution.
    • Flow cytometric analysis of permeabilized HeLa using ab166894 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    ab166894 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 17 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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