Overview

  • Product nameAnti-CatSper2 antibody
    See all CatSper2 primary antibodies
  • Description
    Rabbit polyclonal to CatSper2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A region within synthetic peptide: QAVPRHTIRE LLDPSRQKKL VLGDQHQLVR FSIKPQRIEQ ISHAQRLLSR, corresponding to N terminal amino acids 37-86 of Human CatSper2

  • Positive control
    • IHC-P: Human brain tissue. WB: Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 48 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • RelevanceCatSper2 belongs to a family of putative cation channels that are specific to spermatozoa and localize to the flagellum. Calcium ions play a primary role in the regulation of sperm motility. The protein family features a single repeat with six membrane spanning segments and a predicted calcium selective pore region. A second, closely linked copy of the gene has been identified. Although multiple transcript variants encoding protein isoforms have been characterized, they seem to be only transcribed from this gene. Additional splice variants have been described but their full length nature has not been determined.
  • Cellular localizationCell projection, cilium, flagellum; Multi-pass membrane protein
  • Database links
  • Alternative names
    • Cation channel sperm associated 2 antibody
    • Cation channel sperm associated protein 2 antibody
    • Catsper 2 antibody
    • MGC33346 antibody
    • Putative ion channel protein CATSPER2 variant 1 antibody
    • Putative ion channel protein CATSPER2 variant 2 antibody
    • Putative ion channel protein CATSPER2 variant 3 antibody
    • Sperm associated cation channel 2 antibody
    • Sperm ion channel antibody
    see all

Anti-CatSper2 antibody images

  • Anti-CatSper2 antibody (ab49341) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • ab49341 at a concentration of 4-8 µg/ml positively labelling neural cells in human brain tissue (indicated by arrows). Magnification 400X

References for Anti-CatSper2 antibody (ab49341)

ab49341 has not yet been referenced specifically in any publications.

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The antibodies ab1828, ab23706 and ab50693 are tested with Bovine (Cow) tissue and are fully guaranteed so the Abreview discount is not valid for these.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"