Overview

  • Product name
  • Description
    Rabbit polyclonal to CATSPERB
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 108-176 of Human CATSPERB.

  • Positive control
    • Human pancreas tissue.
  • General notes
    The antibody solution should be gently mixed before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab150837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Relevance
    RelevanceCATSPERB is a component of a complex composed at least of CATSPER1, CATSPERB, CATSPERG and HSPA1B. CATSPERB contains at least 2 transmembrane domains and 8 N-glycosylation sites. CATSPERB is required for trafficking or formation of a stable channel complex. It is involved in sperm cell hyperactivation via its association with CATSPER1. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization. There are 2 isoforms produced by alternative splicing.
  • Cellular localization
    Membrane; Multi-pass membrane protein
  • Database links
  • Alternative names
    • C14orf161 antibody
    • Cation channel sperm-associated protein subunit beta antibody
    • Cation channel, sperm-associated, beta antibody
    • Catsper channel auxiliary subunit beta antibody
    • CatSper(beta) antibody
    • CATSPERB antibody
    • FLJ14298 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab150837 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CATSPERB with ab150837 antibody at 1/200 dilution.

References

ab150837 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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