The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 245 kDa.
Use a concentration of 0.1 - 1 µg/ml.
Use a concentration of 1 - 10 µg/ml.
Use 0.5-1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
Expressed in pancreatic islets and in brain, where it has been seen in cerebral cortex, hippocampus, basal ganglia, habenula and thalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in skeletal muscle.
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1D subfamily.
Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
ab85491 staining CaV1.3 in mouse backskin tissue by IHC-P (Bouin's fixed paraffin embedded tissue sections). Tissue underwent antigen retrieval using microwave in citrate buffer. The primary antibody was used at 1/100 dilution and then sections were incubated with Fluorophore conjugated goat anti mouse at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CaV1.3 antibody [L48A/9] (ab85491)Image courtesy of an anonymous Abreview.
ab85491 staining CaV1.3 in human differentiated iPS cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.05% Tween-20 and then blocked using 3% BSA for 1 hour at 21°C. Samples were then incubated with primary antibody at a 1/100 dilution for 12 hours at 4°C. The secondary antibody used was a anti-mouse IgG (H+L) conjugated to Alexa Fluor® 594 (pink) used at a 1/400 dilution.
Overlay histogram showing SH-SY5Y cells stained with ab85491 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85491, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
Doshina A et al. Cortical cells reveal APP as a new player in the regulation of GABAergic neurotransmission. Sci Rep7:370 (2017).
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Hammarsten P et al. High Caveolin-1 Expression in Tumor Stroma Is Associated with a Favourable Outcome in Prostate Cancer Patients Managed by Watchful Waiting. PLoS One11:e0164016 (2016).
Read more (PubMed: 27764093) »