1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/20 - 1/100.
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
Expressed in muscle and lung, less so in liver, brain and kidney.
Involvement in disease
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
Belongs to the caveolin family.
The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
Immunohistochemical analysis of formalin-fixed, paraffin embedded Human lung carcinoma tissue labeling Caveolin-1 with ab214448 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Western blot - Anti-Caveolin-1 antibody (ab214448)
All lanes : Anti-Caveolin-1 antibody (ab214448) at 1/200 dilution
Lane 1 : Mouse lung tissue lysate Lane 2 : Mouse muscle tissue lysate
Secondary All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Immunohistochemical analysis of formalin-fixed, paraffin embedded Mouse eye tissue labeling Caveolin-1 with ab214448 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Flow cytometric analysis of Rat RSC96 cells labeling Caveolin-1 with ab214448 at 1/100 dilution followed by conjugated secondary (PE Conjugated) (green) compared to control cells (blue), secondary only (light blue) and isotype control (orange).