Overview

  • Product name
  • Description
    Rabbit polyclonal to Caveolin-1
  • Specificity
    Detects caveolin-1.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Goat, Hamster, Dog, Human
    Predicted to work with: Sheep, Rabbit, Horse, Cow, Cat, Pig, Chimpanzee, Gorilla, African green monkey, African bush elephant
  • Immunogen

    Synthetic peptide corresponding to Human Caveolin-1 aa 1-17.
    Sequence:

    MSGGKYVDSEGHLYTVP


    Database link: Q03135
    (Peptide available as ab4928)

  • Positive control
    • Human lung, heart or spleen
  • General notes

    This antibody can be used as a marker for lipid raft fractions.

Properties

Applications

Our Abpromise guarantee covers the use of ab2910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500. PubMed: 17502389
IHC-Fr Use at an assay dependent concentration. PubMed: 20351069
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Caveolin-1 peptide (ab4928).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • Tissue specificity
    Expressed in muscle and lung, less so in liver, brain and kidney.
  • Involvement in disease
    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • Sequence similarities
    Belongs to the caveolin family.
  • Post-translational
    modifications
    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • Cellular localization
    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Information by UniProt
  • Database links
  • Alternative names
    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

Images

  • All lanes : Anti-Caveolin-1 antibody (ab2910) at 1.5 µg/ml

    Lane 1 : Human lung
    Lane 2 : Human heart
    Lane 3 : Human spleen

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa Fluor anti-rabbit at 1/5000 dilution

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded human cardiac tissue, labelling Caveolin 1 with ab2910 at a dilution of 1/10000 incubated for 2 hours at 21°C in TBS / BSA / Aside solution. Heat mediated antigen retrival was performed with citric acid. Blocking was with 1% BSA incubated for 10 minutes at 21°C. Secondary used was a goat anti-rabbit polyclonal biotin conjugate at 1/300.Image shows strong immunopositivity at the membranes of cardiomyocytes ( invaginations of immunostaining show points of branching of each myocyte). There is intense positivity in the smooth muscle of of arterioles. Not seen is the intense positivity of what seems to be the endothelial lining cells of the endocardium.

    See Abreview

  • Rat astrocytes stained with fluorescently labelled Caveolin-1 antibody. Primary antibody is ab2910 (dilution 1/500) and the secondary antibody is Texas red labelled anti-rabbit IgG (dilution1/1000).

    This image was kindly supplied as part of the review submitted by Donghui Zhu.

  • ab2910 at 1/250 dilution staining mouse NIH 3T3 cells by Immunocytochemistry (panel B). The antibody was incubated with the paraformaldehyde fixed cells for 12 hours. Bound antibody was detected using an Alexa Fluor ® 594 conjugated Goat anti-rabbit antibody (ab150080). Panel A shows staining with a mouse anti-Caveolin 1 antibody (clone 2297). Panel C shows the merged image.

    This image is courtesy of an Abreview by William Ackerman.

    See Abreview

  • ab2910 staining Caveolin-1 - Caveolae Marker in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/200 in PBS + 0.05% Saponin) for 1 hour at 37°C. A Cy3®-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab2910 diluted 1/500 and was incubated with A549 whole cell lysate and a Protein A/G matrix for 16 hours at 4°C to achieve immunoprecipitation.  400 µg of lysate was present in the input.

    An HRP-conjugated goat anti-rabbit was used for the Western Blot step.

    Lane 1: Whole Cell

    Lane 2: IP-cav-1

    Lane 3: Unrelated antibody

    Lane 4: Unrelated antibody

    See Abreview



  • Predicted band size : 20 kDa

    Western blot of caveolin-1 on rat heart protein extract using ab2910.

References

This product has been referenced in:
  • Shen Y  et al. Flagellar Hooks and Hook Protein FlgE Participate in Host Microbe Interactions at Immunological Level. Sci Rep 7:1433 (2017). WB ; Mouse . Read more (PubMed: 28469201) »
  • Li M  et al. Caveolin1 protects against diet induced hepatic lipid accumulation in mice. PLoS One 12:e0178748 (2017). Read more (PubMed: 28570612) »

See all 57 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
50 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Juan Rodríguez Vita

Verified customer

Submitted Mar 27 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3 fibroblasts)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
10 µg
Specification
3T3 fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Goat Tissue sections (Myocardium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Myocardium
Blocking step
(agent) for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Jan 28 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (neuron)
Permeabilization
Yes - 0.2%Triton
Specification
neuron
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 13 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS-7)
Permeabilization
Yes - 0.2%Triton
Specification
COS-7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Username

Ms. Ya Zhou

Verified customer

Submitted Oct 06 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (Heart muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Heart muscle
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Heart muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Heart muscle
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Heart muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Heart muscle
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Marmoset (common) Tissue sections (Heart muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Heart muscle
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Heart
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Sep 21 2015

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