Our Abpromise guarantee covers the use of ab18199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).|
ICC/IF image of ab18199 stained NIH/3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18199, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
ab18199 staining human HT1080 cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to incubating with ab18199 at 10 µg/ml for 1 hour at 20°C. A Texas Red® conjugated donkey anti-rabbit antibody was used as the secondary.