Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)


  • Product nameAnti-Caveolin-1 antibody [E249] - Caveolae Marker
    See all Caveolin-1 primary antibodies
  • Description
    Rabbit monoclonal [E249] to Caveolin-1 - Caveolae Marker
  • SpecificityThis antibody should recognize both alpha and beta form of Caveolin-1.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, ICC, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caveolin-1 aa 150-250.
    Database link: Q03135

  • Positive control
    • A431 cell lysate, human urinary bladder tissue,human lung tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Alternative versions available:

    Anti-Caveolin-1 antibody (Alexa Fluor® 488) [E249] - Caveolae Marker (ab185043)

    Anti-Caveolin-1 antibody (Alexa Fluor® 647) [E249] - Caveolae Marker (ab193892)

    Anti-Caveolin-1 antibody (HRP) [E249] - Caveolae Marker (ab193893)



Our Abpromise guarantee covers the use of ab32577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19808975
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 0.01M Sodium Citrate Buffer, pH 6.0.
ICC 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionMay act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
    • Tissue specificityExpressed in muscle and lung, less so in liver, brain and kidney.
    • Involvement in diseaseDefects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
    • Sequence similaritiesBelongs to the caveolin family.
    • Post-translational
      The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
    • Cellular localizationGolgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
    • Information by UniProt
    • Database links
    • Alternative names
      • BSCL3 antibody
      • CAV antibody
      • CAV1 antibody
      • CAV1_HUMAN antibody
      • caveolae protein, 22 kD antibody
      • caveolin 1 alpha isoform antibody
      • caveolin 1 beta isoform antibody
      • Caveolin 1 caveolae protein 22kDa antibody
      • Caveolin-1 antibody
      • Caveolin1 antibody
      • cell growth-inhibiting protein 32 antibody
      • CGL3 antibody
      • LCCNS antibody
      • MSTP085 antibody
      • OTTHUMP00000025031 antibody
      • PPH3 antibody
      • VIP 21 antibody
      • VIP21 antibody
      see all

    Anti-Caveolin-1 antibody [E249] - Caveolae Marker images

    • Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577) + A431 cell lysate

      Predicted band size : 20 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human urinary bladder tissue, staining Caveolin-1 with ab32577 at 1/250 dilution.

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung tissue, staining Caveolin-1 with ab32577 at 1/250 µg/ml.

    • Immunohistochemical analysis of developing Human lung tissue, staining Caveolin-1 with ab32577 at 1/250 dilution.

    References for Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)

    This product has been referenced in:
    • Qayyum T  et al. The interrelationships between Src, Cav-1 and RhoGD12 in transitional cell carcinoma of the bladder. Br J Cancer 106:1187-95 (2012). Read more (PubMed: 22353809) »
    • Kaarteenaho R  et al. Epithelial N-cadherin and nuclear ß-catenin are up-regulated during early development of human lung. BMC Dev Biol 10:113 (2010). IHC-P ; Human . Read more (PubMed: 21080917) »

    See all 4 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Kidney)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
    Permeabilization Yes - Wash Buffer from Dako with Tween
    Specification Kidney
    Fixative Paraformaldehyde

    Mr. Rudolf Jung

    Verified customer

    Submitted Jun 17 2016

    Thank you for contacting us.

    Regarding antibodies ab79397 and ab32577, they both have been tested in IHC with paraffin embedded sections in the species stated on the datasheet. We don’t have preliminary data using them with frozen secti...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HUVEC)
    Loading amount 100000 cells
    Specification HUVEC
    Treatment various doses of VEGF for 24h
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted May 05 2008