Anti-Caveolin-1 antibody - N-terminal (ab211503)
Key features and details
- Goat polyclonal to Caveolin-1 - N-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
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Overview
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Product name
Anti-Caveolin-1 antibody - N-terminal
See all Caveolin-1 primary antibodies -
Description
Goat polyclonal to Caveolin-1 - N-terminal -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Monkey -
Immunogen
Recombinant fragment within Human Caveolin-1 aa 1-100 (N terminal). The exact sequence is proprietary. (NP_001744.2).
Database link: Q03135 -
Positive control
- HaCat, MDCK and 3T3 cell lysates; Human heart tissue; Hepa1-6 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 20% Glycerol (glycerin, glycerine), 79% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/500 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 20 kDa).
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ICC/IF |
1/25 - 1/250.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/500 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 20 kDa). |
ICC/IF
1/25 - 1/250. |
Target
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Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. -
Tissue specificity
Expressed in muscle and lung, less so in liver, brain and kidney. -
Involvement in disease
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. -
Sequence similarities
Belongs to the caveolin family. -
Post-translational
modificationsThe initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
Cellular localization
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. - Information by UniProt
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Database links
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Omim: 601047 Human
- SwissProt: Q03135 Human
- SwissProt: P49817 Mouse
- Unigene: 74034 Human
- Unigene: 28278 Mouse
- Unigene: 470537 Mouse
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Alternative names
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
Images
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Anti-Caveolin-1 antibody - N-terminal (ab211503) at 1/1000 dilution + HaCat cell lysate at 100 µg
Secondary
Rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution
Predicted band size: 20 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue labeling Caveolin-1 with ab211503 at 5 µg/ml.
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Immunofluorescent analysis of 4% PFA-fixed Hepa1-6 cells labeling Caveolin-1 with ab211503 at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab211503 has been referenced in 1 publication.
- Peng H et al. Caveolin-1 Is Essential for the Improvement of Insulin Sensitivity through AKT Activation during Glargine Treatment on Diabetic Mice. J Diabetes Res 2021:9943344 (2021). PubMed: 34917687