Overview

  • Product nameAnti-Caveolin-2 antibody
    See all Caveolin-2 primary antibodies
  • Description
    Rabbit polyclonal to Caveolin-2
  • SpecificityDetects caveolin-2 from rat tissues. This antibody does not detect caveolin-1 or -3.
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Sheep, Rabbit, Cow, Cat, Pig, Chimpanzee, Gorilla, African Green Monkey, Loxodonta Africana (African bush elephant)
  • Immunogen

    Synthetic peptide corresponding to Rat Caveolin-2 aa 1-19.
    Sequence:

    MGLETEKADVQLFMADDAY


    Database link: Q2IBC5
    (Peptide available as ab4929)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Can be blocked with Caveolin-2 peptide (ab4929).

Can be blocked with Caveolin-2 peptide.

IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 20130741
ICC/IF 1/200.

Target

  • FunctionMay act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
  • Tissue specificityExpressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
  • Sequence similaritiesBelongs to the caveolin family.
  • Post-translational
    modifications
    Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
  • Cellular localizationNucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAV antibody
    • CAV2 antibody
    • CAV2_HUMAN antibody
    • Caveolae protein 20 kD antibody
    • Caveolin 2 antibody
    • Caveolin 2 isoform a and b antibody
    • Caveolin-2 antibody
    • MGC12294 antibody
    • OTTHUMP00000025032 antibody
    • OTTHUMP00000195982 antibody
    see all

Anti-Caveolin-2 antibody images

  •  Western blot of caveolin-2 on rat skeletal muscle protein extract using ab2911.

  • IHC image of ab2911 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2911, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Caveolin-2 antibody (ab2911)

This product has been referenced in:
  • Hoeke L  et al. Intestinal Salmonella typhimurium infection leads to miR-29a induced caveolin 2 regulation. PLoS One 8:e67300 (2013). WB . Read more (PubMed: 23826261) »
  • Cho WJ  et al. Caveolin-1 exists and may function in cardiomyocytes. Can J Physiol Pharmacol 88:73-6 (2010). IHC-P ; Mouse . Read more (PubMed: 20130741) »

See all 7 Publications for this product

Product Wall

Thank you for your enquiry.

I am sorry to confirm that regrettablythe KD values for these two antibodies, ab2910 and ab2911, has not been assessed and so we are unable to provide this information. It is unusual for the KD values to be assess...

Read More

Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -...

Read More
Application Western blot
Sample Pig Tissue lysate - whole (Colon)
Loading amount 20 µg
Specification Colon
Gel Running Conditions Reduced Denaturing (Trizingel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2%
Username

Abcam user community

Verified customer

Submitted Sep 29 2011

Application Western blot
Sample Human Cell lysate - whole cell (HEK 293, Kidney)
Loading amount 100 µg
Specification HEK 293, Kidney
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Olga Roche

Verified customer

Submitted Dec 19 2007

According to the datasheet, rat heart and skeletal muscle was used to test this antibody, so you can use it as positive control. We are sorry but we do not sell the immunizing peptide. We would suggest looking at the following reference, please tak...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"