Overview

  • Product nameAnti-Caveolin-2 antibody
    See all Caveolin-2 primary antibodies
  • Description
    Rabbit polyclonal to Caveolin-2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Cat, Pig, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Caveolin-2 aa 2-15.
    Sequence:

    GLETEKADVQLFMD


    Database link: P51636

  • Positive control
    • WB: A549 and HCT116 cell lysate ICC/IF: A431 cell IHC-P: FaDu xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab97476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • FunctionMay act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
  • Tissue specificityExpressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
  • Sequence similaritiesBelongs to the caveolin family.
  • Post-translational
    modifications
    Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
  • Cellular localizationNucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAV antibody
    • CAV2 antibody
    • CAV2_HUMAN antibody
    • Caveolae protein 20 kD antibody
    • Caveolin 2 antibody
    • Caveolin 2 isoform a and b antibody
    • Caveolin-2 antibody
    • MGC12294 antibody
    • OTTHUMP00000025032 antibody
    • OTTHUMP00000195982 antibody
    see all

Anti-Caveolin-2 antibody images

  • Anti-Caveolin-2 antibody (ab97476) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : 18 kDa
    12% SDS PAGE
  • Immunofluorescence analysis of Caveolin-2 in paraformaldehyde fixed A431, using ab97476 at a 1/200 dilution.
    The lower image (2) was co-stained with Hoescht 33342.

  • Immunohistochemical analysis of Caveolin-2 in paraffin embedded FaDu xenograft, using ab97476 at a 1/500 dilution.

References for Anti-Caveolin-2 antibody (ab97476)

ab97476 has not yet been referenced specifically in any publications.

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