Recombinant
RabMAb

Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

Overview

  • Product name
    Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker
    See all Caveolin-2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2220] to Caveolin-2 - Caveolae Marker
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caveolin-2.
    Database link: P51636

  • Positive control
    • WB: HeLa cell lysate. IHC-P: Human uterus tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • General notes

     

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab79397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Predicted molecular weight: 18 kDa.
IP 1/10.
IHC-P 1/100 - 1/250.
Flow Cyt 1/2000.
ICC/IF 1/100 - 1/250.

Target

  • Function
    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
  • Tissue specificity
    Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
  • Sequence similarities
    Belongs to the caveolin family.
  • Post-translational
    modifications
    Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
  • Cellular localization
    Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAV antibody
    • CAV2 antibody
    • CAV2_HUMAN antibody
    • Caveolae protein 20 kD antibody
    • Caveolin 2 antibody
    • Caveolin 2 isoform a and b antibody
    • Caveolin-2 antibody
    • MGC12294 antibody
    • OTTHUMP00000025032 antibody
    • OTTHUMP00000195982 antibody
    see all

Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker images

  • Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397) at 1/5000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 18 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  • ab79397 at 1/100 dilution staining Caveolin-2 in human uterus by Immunohistochemistry using paraffin-embedded tissue. Note staining of endothelial cells and smooth muscles.

  • ab79397 at 1/100 dilution staining Caveolin-2 in HeLa cells by Immunofluorescence.

  • Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caveolin-2 (red) with ab79397 at a 1/2000 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

References for Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

ab79397 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Regarding antibodies ab79397 and ab32577, they both have been tested in IHC with paraffin embedded sections in the species stated on the datasheet. We don’t have preliminary data using them with frozen secti...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up