Overview

  • Product nameAnti-Caveolin-2 (phospho Y19) antibody
    See all Caveolin-2 primary antibodies
  • Description
    Rabbit polyclonal to Caveolin-2 (phospho Y19)
  • SpecificityDetects phospho-caveolin-2 phosphorylated on Tyr 19.
  • Tested applicationsSuitable for: ICC, WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide corresponding to Mouse Caveolin-2 aa 14-25.
    Sequence: MADDAYpSHHSGC
    (Peptide available as ab4962)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/10 - 1/100.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 21 kDa.Can be blocked with Mouse Caveolin-2 peptide (ab4962).
IP Use at an assay dependent concentration.
ICC/IF 1/10 - 1/100.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
  • Tissue specificityExpressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
  • Sequence similaritiesBelongs to the caveolin family.
  • Post-translational
    modifications
    Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
  • Cellular localizationNucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAV antibody
    • CAV2 antibody
    • CAV2_HUMAN antibody
    • Caveolae protein 20 kD antibody
    • Caveolin 2 antibody
    • Caveolin 2 isoform a and b antibody
    • Caveolin-2 antibody
    • MGC12294 antibody
    • OTTHUMP00000025032 antibody
    • OTTHUMP00000195982 antibody
    see all

Anti-Caveolin-2 (phospho Y19) antibody images

  • Immunocytochemistry/Immunofluorescence analysis of Phospho-Caveolin-2 pTyr19 (green) showing staining in the cytoplasm and nucleus of HUVEC cells treated with 100µM pervanadate (left) and untreated HUVEC cells (right). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3417 in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • IHC image of ab3417 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3417, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab3417 at a 1/250 dilution staining Caveolin-2 in rat fibroblasts by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized with Triton X-100 and blocked using 1% BSA. The secondary used was a TRITC conjugated anti-rabbit at a 1/100 dilution.(a) Control cells untreated cells (b) 100nM Insulin for 10 min (c) 10uM U0126 for 2 hr and 100nM Insulin for 10 min (d) 100 nM Wortmannin for 1hr and 100nM Insulin for 10 min

References for Anti-Caveolin-2 (phospho Y19) antibody (ab3417)

This product has been referenced in:
  • Abel B  et al. N-terminal tyrosine phosphorylation of caveolin-2 negates anti-proliferative effect of transforming growth factor beta in endothelial cells. FEBS Lett 586:3317-23 (2012). Read more (PubMed: 22819829) »
  • Kwon H  et al. Caveolin-2 regulation of STAT3 transcriptional activation in response to insulin. Biochim Biophys Acta 1793:1325-33 (2009). WB ; Rat . Read more (PubMed: 19427337) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Cell lysate - whole cell (Fibroblasts)
Total protein in input 1000 µg
Specification Fibroblasts
Treatment 100 nM insulin for 10 min
Immuno-precipitation step Protein A
Username

Dr. Y Pak

Verified customer

Submitted Mar 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Fibroblasts)
Specification Fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Username

Dr. Y Pak

Verified customer

Submitted Mar 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Fibroblasts)
Loading amount 50 µg
Specification Fibroblasts
Treatment 100 nM insulin for 10, 30, 60, 120, and 180 min
Gel Running Conditions Reduced Denaturing (15% gel)
Blocking step BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Y Pak

Verified customer

Submitted Aug 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat)
Loading amount 1e+006 cells
Specification Jurkat
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Jul 28 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"