Recombinant
RabMAb

Anti-Caveolin-2 (phospho Y19) antibody [EPR2220(3)] (ab124883)

Overview

  • Product name
    Anti-Caveolin-2 (phospho Y19) antibody [EPR2220(3)]
    See all Caveolin-2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2220(3)] to Caveolin-2 (phospho Y19)
  • Specificity
    ab124883 detects Caveolin-2 phosphorylated at tyrosine 19.
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caveolin-2 aa 1-100.
    Database link: P51636

  • Positive control
    • HUVEC cell lysate treated with Pervanadate; HUVEC cells treated with Pervanadate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 18 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
    • Tissue specificity
      Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
    • Sequence similarities
      Belongs to the caveolin family.
    • Post-translational
      modifications
      Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
    • Cellular localization
      Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
    • Information by UniProt
    • Database links
    • Alternative names
      • CAV antibody
      • CAV2 antibody
      • CAV2_HUMAN antibody
      • Caveolae protein 20 kD antibody
      • Caveolin 2 antibody
      • Caveolin 2 isoform a and b antibody
      • Caveolin-2 antibody
      • MGC12294 antibody
      • OTTHUMP00000025032 antibody
      • OTTHUMP00000195982 antibody
      see all

    Images

    • All lanes : Anti-Caveolin-2 (phospho Y19) antibody [EPR2220(3)] (ab124883) at 1/1000 dilution

      Lane 1 : HUVEC cell lysate untreated
      Lane 2 : HUVEC cell lysate treated with Pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 18 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized HUVEC cells, untreated (green) or pervanadate treated (red) using ab124883 at 1/10 dilution, and pervanadate-treated HUVEC cells using the same antibody preincubated with phospho-Caveolin 2 Phospho (pY19) peptide (blue) or non-phospho- Caveolin-2 Phospho (pY19) peptide (orange).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124883 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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