Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y] (ab76405)

Overview

  • Product nameAnti-Caveolin-2 (phospho Y27) antibody [EP2221Y]
    See all Caveolin-2 primary antibodies
  • Description
    Rabbit monoclonal [EP2221Y] to Caveolin-2 (phospho Y27)
  • SpecificityThis antibody detects Caveolin-2 phosphorylated at tyrosine 27.
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caveolin-2.
    Database link: P51636

  • Positive control
    • Hela cell lysates
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 18 kDa.
IP 1/40.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • FunctionMay act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
    • Tissue specificityExpressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
    • Sequence similaritiesBelongs to the caveolin family.
    • Post-translational
      modifications
      Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
    • Cellular localizationNucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
    • Information by UniProt
    • Database links
    • Alternative names
      • CAV antibody
      • CAV2 antibody
      • CAV2_HUMAN antibody
      • Caveolae protein 20 kD antibody
      • Caveolin 2 antibody
      • Caveolin 2 isoform a and b antibody
      • Caveolin-2 antibody
      • MGC12294 antibody
      • OTTHUMP00000025032 antibody
      • OTTHUMP00000195982 antibody
      see all

    Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y] images

    • All lanes : Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y] (ab76405) at 1/2000 dilution

      Lane 1 : HeLa cell lysates untreated.
      Lane 2 : HeLa cell lysates treated with pervanadate.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 18 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)

    References for Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y] (ab76405)

    ab76405 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"