Anti-CBARA1 antibody (ab102830)

Overview

  • Product nameAnti-CBARA1 antibody
    See all CBARA1 primary antibodies
  • Description
    Rabbit polyclonal to CBARA1
  • SpecificityThis antibody is expected to recognize isoform 1 and 2.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a sequence between N terminal amino acids 59-89 of Human CBARA1.

  • Positive control
    • K562 cell line lysate IF/ICC: HepG2 cell line

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab102830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 54 kDa.

Target

  • FunctionKey regulator of mitochondrial calcium uptake required for calcium entry into mitochondrion. May act as a calcium sensor via its EF-hand domains, gating the activity of a calcium channel partner. Induces T-helper 1-mediated autoreactivity, which is accompanied by the release of IFNG.
  • Tissue specificityExpressed in epithelial cell line. Strongly expressed in epidermal keratinocytes and dermal endothelial cells.
  • Sequence similaritiesBelongs to the MICU1 family.
    Contains 2 EF-hand domains.
  • DomainThe EF-hand domains are required for mitochondrial calcium homeostasis function.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Allergen Hom s 4 antibody
    • ara CALC antibody
    • atopy related autoantigen antibody
    • atopy related autoantigen CALC antibody
    • Atopy-related autoantigen CALC antibody
    • CALC antibody
    • calcium binding atopy related autoantigen 1 antibody
    • Calcium uptake protein 1 antibody
    • calcium uptake protein 1, mitochondrial antibody
    • Calcium-binding atopy-related autoantigen 1 antibody
    • CBARA1 antibody
    • EFHA3 antibody
    • FLJ12684 antibody
    • MICU1 antibody
    • MICU1_HUMAN antibody
    • mitochondrial antibody
    • mitochondrial calcium uptake 1 antibody
    • MPXPS antibody
    see all

Anti-CBARA1 antibody images

  • ICC/IF image of ab102830 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab102830, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CBARA1 antibody (ab102830) at 1/100 dilution + K562 cell line lysate at 35 µg

    Predicted band size : 54 kDa

References for Anti-CBARA1 antibody (ab102830)

ab102830 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I am sorry that these antibodies did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passin...

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Thank you for submitting an Abreview for ab102830. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested species/application, we would like to follow up ...

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - other (rat brain mitochondria)
Loading amount 35 µg
Specification rat brain mitochondria
Gel Running Conditions Non-reduced Denaturing
Blocking step StartingBlock T20 (PBS) Blocking Buffer as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Username

Dr. Alexander Komarov

Verified customer

Submitted Aug 24 2012

Thank you for this information.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab 135358.

To check the sta...

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Thank you for your reply.


The immunogen sequence used to generate this antibody (ab102830) is excluded from this isoform, so this is not a possibility. Ab115025 would recognize this isoform, but itis not present on your blot.

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Thank you for your reply.


Yes, this antibody is detecting Isoform 4 (MW 32kDa), as opposed to the other two which detected isoform 1 in the samples. You may still detect a 55kDa band in your samples if that is the isoform present.
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Thank you for contacting Abcam regarding these CBARA1 antibodies.


Based on the information and images you provided, I agree that these antibodies are not performing as we would expect. I am happy to offer replacements or refunds for both ...

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I am very pleased to hear you would like to accept our offer and test ab102830 in rat. This code will give you: 1 freePRIMARY ANTIBODYOR $337.00 OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for ab102830 ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"