Overview

  • Product name
  • Description
    Mouse polyclonal to CBF
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length human CBF protein (NP_005751.2).

  • Positive control
    • WB: NIH/3T3 cell lysate; K-562 cell lysate; CBF transfected 293T cell lysate ICC/IF: HeLa cell

Properties

Applications

Our Abpromise guarantee covers the use of ab89034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 121 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

Images

  • Anti-CBF antibody (ab89034) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg

    Predicted band size : 121 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • Anti-CBF antibody (ab89034) at 1 µg/ml + K-562 cell lysate at 50 µg

    Predicted band size : 121 kDa
    Observed band size : 131 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-CBF antibody (ab89034) at 1 µg/ml

    Lane 1 : CEBPZ transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 121 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
  • ab89034, at 10 µg/ml, staining CBF in Hela cell by Immunofluorescence.

References

ab89034 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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