Anti-CBF1 interacting corepressor/CIR1 antibody (ab113203)
Key features and details
- Rabbit polyclonal to CBF1 interacting corepressor/CIR1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CBF1 interacting corepressor/CIR1 antibody
See all CBF1 interacting corepressor/CIR1 primary antibodies -
Description
Rabbit polyclonal to CBF1 interacting corepressor/CIR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee -
Immunogen
Synthetic peptide corresponding to Human CBF1 interacting corepressor/CIR1 aa 400-500.
Database link: Q86X95 -
Positive control
- HeLa, Jurkat and 293T cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99.91% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 52 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Relevance
CBF1 interacting corepressor may modulate splice site selection during alternative splicing of pre-mRNAs. It regulates transcription and acts as corepressor for RBPSUH; recruits RBPSUH to the Sin3-histone deacetylase complex (HDAC). -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 9541 Human
- Omim: 605228 Human
- SwissProt: Q86X95 Human
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Alternative names
- CIR antibody
- Recepin antibody
Images
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All lanes : Anti-CBF1 interacting corepressor/CIR1 antibody (ab113203) at 0.1 µg/ml
Lane 1 : HeLa cell Lysate at 50 µg
Lane 2 : HeLa cell Lysate at 15 µg
Lane 3 : Jurkat cell Lysate at 50 µg
Lane 4 : 293T cell Lysate at 50 µg
Predicted band size: 52 kDa
Exposure time: 30 seconds -
Detection of CBF1 interacting corepressor/CIR1 by Western Blot of Immunprecipitate.
ab113203 at 1µg/ml staining CBF1 interacting corepressor/CIR1 in HeLa whole cell lysate immunoprecipitated using ab113203 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab113203 has not yet been referenced specifically in any publications.