Anti-CBF1 interacting corepressor antibody (ab113203)

Overview

  • Product name
    Anti-CBF1 interacting corepressor antibody
  • Description
    Rabbit polyclonal to CBF1 interacting corepressor
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 400-450 of Human CBF1 interacting corepressor (NP_004873.3)

  • Positive control
    • HeLa, Jurkat and 293T cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab113203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance
    CBF1 interacting corepressor may modulate splice site selection during alternative splicing of pre-mRNAs. It regulates transcription and acts as corepressor for RBPSUH; recruits RBPSUH to the Sin3-histone deacetylase complex (HDAC).
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • CIR antibody
    • Recepin antibody

Images

  • All lanes : Anti-CBF1 interacting corepressor antibody (ab113203) at 0.1 µg/ml

    Lane 1 : HeLa cell Lysate at 50 µg
    Lane 2 : HeLa cell Lysate at 15 µg
    Lane 3 : Jurkat cell Lysate at 50 µg
    Lane 4 : 293T cell Lysate at 50 µg


    Predicted band size : 52 kDa


    Exposure time : 30 seconds
  • Detection of CBF1 interacting corepressor by Western Blot of Immunprecipitate.
    ab113203 at 1µg/ml staining CBF1 interacting corepressor in HeLa whole cell lysate immunoprecipitated using ab113203 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.

References

ab113203 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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