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Rabbit polyclonal to CBF1 interacting corepressor
Predicted to work with:
Synthetic peptide, corresponding to a region within amino acids 400-450 of Human CBF1 interacting corepressor (NP_004873.3)
HeLa, Jurkat and 293T cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99.91% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
Use at 2-10 µg/mg of lysate.
CBF1 interacting corepressor may modulate splice site selection during alternative splicing of pre-mRNAs. It regulates transcription and acts as corepressor for RBPSUH; recruits RBPSUH to the Sin3-histone deacetylase complex (HDAC).
Western blot - Anti-CBF1 interacting corepressor antibody (ab113203)
All lanes : Anti-CBF1 interacting corepressor antibody (ab113203) at 0.1 µg/ml Lane 1 : HeLa cell Lysate at 50 µg Lane 2 : HeLa cell Lysate at 15 µg Lane 3 : Jurkat cell Lysate at 50 µg Lane 4 : 293T cell Lysate at 50 µg Predicted band size: 52 kDa Exposure time: 30 seconds
Immunoprecipitation - CBF1 interacting corepressor antibody (ab113203)
Detection of CBF1 interacting corepressor by Western Blot of Immunprecipitate.
ab113203 at 1µg/ml staining CBF1 interacting corepressor in HeLa whole cell lysate immunoprecipitated using ab113203 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
has not yet been referenced specifically in any publications.
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