The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use at an assay dependent concentration. PubMed: 23045605
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 71 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionFunctions as a transcriptional repressor. Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes. Plays a role in granulocyte differentiation. Isoform 2 functions as an A-kinase-anchoring protein.
Tissue specificityWidely expressed with higher expression in heart, pancreas, skeletal muscle, spleen, thymus and peripheral blood leukocytes. Expressed in hematopoietic cells (at protein level).
Involvement in diseaseNote=A chromosomal aberration involving CBFA2T3 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
Sequence similaritiesBelongs to the CBFA2T family. Contains 1 MYND-type zinc finger. Contains 1 TAFH (NHR1) domain.
Cellular localizationNucleus > nucleolus. The RUNX1-CBFA2T3 fusion protein localizes to the nucleoplasm and Nucleus > nucleoplasm. Golgi apparatus membrane.
ICC/IF image of ab33072 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab33072 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - CBFA2T3 antibody (ab33072)
Anti-CBFA2T3 antibody - ChIP Grade (ab33072) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate
Secondary HRP conjugated anti Rabbit IgG at 1/50000 dilution
References for Anti-CBFA2T3 antibody - ChIP Grade (ab33072)
This product has been referenced in:
Thiollier C et al. Characterization of novel genomic alterations and therapeutic approaches using acute megakaryoblastic leukemia xenograft models. J Exp Med209:2017-31 (2012).
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