• Product nameAnti-CBFA2T3 antibody - ChIP Grade
    See all CBFA2T3 primary antibodies
  • Description
    Rabbit polyclonal to CBFA2T3 - ChIP Grade
  • Tested applicationsSuitable for: ICC/IF, ChIP, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster
  • Immunogen

    Synthetic peptide derived from residues 11 - 60 of human CBFA2T3.


  • Positive control
    • Jurkat cell lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: DU145.



Our Abpromise guarantee covers the use of ab33072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 23045605
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 71 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.


  • FunctionFunctions as a transcriptional repressor. Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes. Plays a role in granulocyte differentiation.
    Isoform 2 functions as an A-kinase-anchoring protein.
  • Tissue specificityWidely expressed with higher expression in heart, pancreas, skeletal muscle, spleen, thymus and peripheral blood leukocytes. Expressed in hematopoietic cells (at protein level).
  • Involvement in diseaseNote=A chromosomal aberration involving CBFA2T3 is found in therapy-related myeloid malignancies. Translocation t(16;21)(q24;q22) that forms a RUNX1-CBFA2T3 fusion protein.
  • Sequence similaritiesBelongs to the CBFA2T family.
    Contains 1 MYND-type zinc finger.
    Contains 1 TAFH (NHR1) domain.
  • Cellular localizationNucleus > nucleolus. The RUNX1-CBFA2T3 fusion protein localizes to the nucleoplasm and Nucleus > nucleoplasm. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cbfa2t3 antibody
    • Ccore binding factor runt domain alpha subunit 2 translocated to 3 antibody
    • ETO2 antibody
    • hMTG16 antibody
    • hMTG16a antibody
    • hMTG16b antibody
    • MTG16 antibody
    • MTG16_HUMAN antibody
    • MTG8 related gene 2 antibody
    • MTG8 related protein MTG16a antibody
    • MTG8 related protein MTG16b antibody
    • MTG8-related protein 2 antibody
    • MTGR2 antibody
    • Myeloid translocation gene 8 and 16b antibody
    • Myeloid translocation gene on chromosome 16 protein antibody
    • Protein CBFA2T3 antibody
    • Zinc finger MYND domain-containing protein 4 antibody
    • ZMYND4 antibody
    see all

Anti-CBFA2T3 antibody - ChIP Grade images

  • ICC/IF image of ab33072 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab33072 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-CBFA2T3 antibody - ChIP Grade (ab33072) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate

    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 71 kDa
    Observed band size : 58,62 kDa (why is the actual band size different from the predicted?)

References for Anti-CBFA2T3 antibody - ChIP Grade (ab33072)

This product has been referenced in:
  • Thiollier C  et al. Characterization of novel genomic alterations and therapeutic approaches using acute megakaryoblastic leukemia xenograft models. J Exp Med 209:2017-31 (2012). IP . Read more (PubMed: 23045605) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Purified protein (293T, MO7e, and CMK)
Loading amount 50 µg
Specification 293T, MO7e, and CMK
Treatment 10 mM Sodium Butyrate for 24 hrs
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (haematopoietic progenitor cell line)
Loading amount 40 µg
Specification haematopoietic progenitor cell line
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 06 2011