Overview

  • Product name
  • Description
    Rabbit polyclonal to CBFb
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Horse, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 166-187 of Human CBFb (NP_074036.1).

  • Positive control
    • Jurkat whole cell lysate HeLa whole cell lysate 293T whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 22 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.
  • Involvement in disease
    Note=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11.
  • Sequence similarities
    Belongs to the CBF-beta family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBF b antibody
    • CBF beta antibody
    • CBF-beta antibody
    • CBFB antibody
    • CBFbeta antibody
    • Core binding factor beta subunit antibody
    • core binding factor subunit beta antibody
    • Core-binding factor subunit beta antibody
    • PEA 2 antibody
    • PEA2 antibody
    • PEA2 beta antibody
    • PEA2-beta antibody
    • PEA2beta antibody
    • PEBB_HUMAN antibody
    • PEBP 2B antibody
    • PEBP2 beta antibody
    • PEBP2-beta antibody
    • PEBP2B antibody
    • PEBP2beta antibody
    • Polyomavirus enhancer binding protein 2 beta subunit antibody
    • Polyomavirus enhancer-binding protein 2 beta subunit antibody
    • SL3 3 enhancer factor 1 beta subunit antibody
    • SL3 3 enhancer factor 1 subunit beta antibody
    • SL3-3 enhancer factor 1 subunit beta antibody
    • SL3/AKV core binding factor beta subunit antibody
    • SL3/AKV core-binding factor beta subunit antibody
    see all

Images

  • All lanes : Anti-CBFb antibody (ab125191) at 0.1 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 50 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 22 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation : 1 mg of Jurkat cell lysate immunoprecipitated with ab125191 at 6 µg/mg lysate; 20% of immunoprecipitate loaded in lane

    Western Blot: CBFb antibody (ab125191) at 1 µg/ml

    developed using the ECL technique

    Exposure time : 30 seconds

References

ab125191 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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