Overview

  • Product name
  • Description
    Rabbit polyclonal to CBFb
  • Specificity
    This antibody detects CBFb.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
    Predicted to work with: Chicken, Xenopus laevis
  • Immunogen

    Synthetic peptide:

    MPRVVPDQRSKFENEEFFRK

    , corresponding to amino acids 1-20 of Human CBFb.

  • Positive control
    • HeLa whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: PBS, 1mg/ml BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500. PubMed: 24648201
WB 1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Target

  • Function
    CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.
  • Involvement in disease
    Note=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11.
  • Sequence similarities
    Belongs to the CBF-beta family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBF b antibody
    • CBF beta antibody
    • CBF-beta antibody
    • CBFB antibody
    • CBFbeta antibody
    • Core binding factor beta subunit antibody
    • core binding factor subunit beta antibody
    • Core-binding factor subunit beta antibody
    • PEA 2 antibody
    • PEA2 antibody
    • PEA2 beta antibody
    • PEA2-beta antibody
    • PEA2beta antibody
    • PEBB_HUMAN antibody
    • PEBP 2B antibody
    • PEBP2 beta antibody
    • PEBP2-beta antibody
    • PEBP2B antibody
    • PEBP2beta antibody
    • Polyomavirus enhancer binding protein 2 beta subunit antibody
    • Polyomavirus enhancer-binding protein 2 beta subunit antibody
    • SL3 3 enhancer factor 1 beta subunit antibody
    • SL3 3 enhancer factor 1 subunit beta antibody
    • SL3-3 enhancer factor 1 subunit beta antibody
    • SL3/AKV core binding factor beta subunit antibody
    • SL3/AKV core-binding factor beta subunit antibody
    see all

References for Anti-CBFb antibody (ab33516)

This product has been referenced in:
  • Cieslak A  et al. RUNX1-dependent RAG1 deposition instigates human TCR-d locus rearrangement. J Exp Med 211:1821-32 (2014). WB, IP ; Human . Read more (PubMed: 25135298) »
  • Lopez-Camacho C  et al. Core binding factor ß (CBFß) is retained in the midbody during cytokinesis. J Cell Physiol 229:1466-74 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24648201) »

See all 7 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic stem cells Strain 129)
Loading amount
35 µg
Specification
Mouse Embryonic stem cells Strain 129
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Oct 04 2010

Application
Western blot
Sample
Rat Cell lysate - whole cell (UMR-106 OSTEOSARCOMA)
Loading amount
50 µg
Specification
UMR-106 OSTEOSARCOMA
Gel Running Conditions
Reduced Denaturing (10% PAGE SDS)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Cesar Camacho

Verified customer

Submitted May 06 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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