Overview

  • Product name
    Anti-CBFb antibody - ChIP Grade
    See all CBFb primary antibodies
  • Description
    Rabbit polyclonal to CBFb - ChIP Grade
  • Host species
    Rabbit
  • Tested applications
    Suitable for: CHIPseq, ChIPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Human CBFb (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: Q13951

    Synthetic peptide corresponding to Human CBFb (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: Q13951

  • Positive control
    • Chromatin from SKNO-1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab195411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
CHIPseq Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.

Use 4 μl per sheared chromatin from 1.25 million cells.

 

Target

  • Function
    CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.
  • Involvement in disease
    Note=A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11.
  • Sequence similarities
    Belongs to the CBF-beta family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBF b antibody
    • CBF beta antibody
    • CBF-beta antibody
    • CBFB antibody
    • CBFbeta antibody
    • Core binding factor beta subunit antibody
    • core binding factor subunit beta antibody
    • Core-binding factor subunit beta antibody
    • PEA 2 antibody
    • PEA2 antibody
    • PEA2 beta antibody
    • PEA2-beta antibody
    • PEA2beta antibody
    • PEBB_HUMAN antibody
    • PEBP 2B antibody
    • PEBP2 beta antibody
    • PEBP2-beta antibody
    • PEBP2B antibody
    • PEBP2beta antibody
    • Polyomavirus enhancer binding protein 2 beta subunit antibody
    • Polyomavirus enhancer-binding protein 2 beta subunit antibody
    • SL3 3 enhancer factor 1 beta subunit antibody
    • SL3 3 enhancer factor 1 subunit beta antibody
    • SL3-3 enhancer factor 1 subunit beta antibody
    • SL3/AKV core binding factor beta subunit antibody
    • SL3/AKV core-binding factor beta subunit antibody
    see all

Images

  • ChIP results obtained with ab195411 directed against CBFb

    ChIP assays were performed using SKNO-1 cells, ab195411 and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 µl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, OGG1, NFE2, and SPI1 genes. Figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.

  • ChIP assays were performed using SKNO-1 cells, ab195411 and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. The IP’d DNA from 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. This figure shows the results of the complete chromosome 3 and three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.

Protocols

References

ab195411 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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