• Product nameAnti-CBL antibody
    See all CBL primary antibodies
  • Description
    Rabbit polyclonal to CBL
  • Specificityab59211 detects endogenous levels of total CBL protein.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human CBL around the phosphorylation site of tyrosine 700 (T-E-YP-M-T).

  • Positive control
    • IHC-P: Human breast carcinoma tissue. WB: 293 cell extracts.



Our Abpromise guarantee covers the use of ab59211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/5000.
    IHC-Paraffin: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionParticipates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
    • PathwayProtein modification; protein ubiquitination.
    • Involvement in diseaseDefects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.
    • Sequence similaritiesContains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
      Contains 1 RING-type zinc finger.
      Contains 1 UBA domain.
    • DomainThe RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
      The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
    • Post-translational
      Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 4732447J05Rik antibody
      • C CBL antibody
      • Cas Br M (murine) ecotropic retroviral transforming sequence antibody
      • Casitas B lineage lymphoma proto oncogene antibody
      • Casitas B-lineage lymphoma proto-oncogene antibody
      • CBL 2 antibody
      • cbl antibody
      • CBL_HUMAN antibody
      • CBL2 antibody
      • E3 ubiquitin protein ligase CBL antibody
      • E3 ubiquitin-protein ligase CBL antibody
      • Oncogene CBL2 antibody
      • Proto oncogene c CBL antibody
      • Proto-oncogene c-CBL antibody
      • RGD1561386 antibody
      • RING finger protein 55 antibody
      • RNF55 antibody
      • Signal transduction protein CBL antibody
      see all

    Anti-CBL antibody images

    • ab59211, at 1/50 - 1/100 dilution, staining CBL in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
    • All lanes : Anti-CBL antibody (ab59211) at 1/500 dilution

      Lane 1 : 293 cell extract
      Lane 2 : 293 cell extract with immunising peptide

      Predicted band size : 99 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)

    References for Anti-CBL antibody (ab59211)

    ab59211 has not yet been referenced specifically in any publications.

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