Overview

  • Product nameAnti-CBLL1 antibody
    See all CBLL1 primary antibodies
  • Description
    Rabbit polyclonal to CBLL1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Dog, Zebrafish
  • Immunogen

    A region within synthetic peptide: FTQPGGMSPG IWPAPRGPPP PPRLQGPPSQ TPLPGPHHPD QTRYRPYYQ, corresponding to C terminal amino acids 443-491 of Human CBLL1

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab50993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

Target

  • FunctionPromotes ubiquitination of tyrosine-phosphorylated CDH1, thus targeting CDH1 for endocytosis and degradation.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 C2H2-type zinc finger.
    Contains 1 RING-type zinc finger.
  • Information by UniProt
  • Database links
  • Alternative names
    • c-Cbl-like protein 1 antibody
    • CasBrM (murine) ecotropic retroviral transforming sequencelike 1 antibody
    • Casitas B-lineage lymphoma transforming sequence-like protein 1 antibody
    • Casitas B-lineage lymphoma-like 1 antibody
    • Casitas B-lineage lymphoma-transforming sequence-like protein 1 antibody
    • cbll1 antibody
    • E3 ubiquitin-protein ligase Hakai antibody
    • Ecadherin binding protein E7 antibody
    • FLJ23109 antibody
    • HAKAI antibody
    • HAKAI_HUMAN antibody
    • MGC163401 antibody
    • MGC163403 antibody
    • OTTHUMP00000206720 antibody
    • OTTHUMP00000206722 antibody
    • OTTHUMP00000206724 antibody
    • RING finger protein 188 antibody
    • RNF188 antibody
    see all

Anti-CBLL1 antibody images

  • Lane 1 : MW marker
    Lane 2 : Anti-CBLL1 antibody (ab50993) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 55 kDa

References for Anti-CBLL1 antibody (ab50993)

ab50993 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (gel 8%)
Sample Human Cell lysate - whole cell (cell line MDCK)
Specification cell line MDCK
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 27°C
Username

宣 若橋

Verified customer

Submitted Jul 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"