Overview

  • Product name
  • Description
    Rabbit polyclonal to CBLN1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human CBLN1 (internal sequence). A 15 amino acid synthetic peptide from the middle of human CBLN1.

  • Positive control
    • Human prostate tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab64184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Function
    Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the formation and maintenance of parallel fiber and Purkinje cell synapses. When parallel fibers make contact with Purkinje spines, CBLN1 interaction with GRID2 triggers the recruitment of NRXN1 and secretory vesicles to the sites of contact. NRXN1-CBLN1-GRID2 signaling induces presynaptic morphological changes, which may further accumulate pre- and postsynaptic components to promote bidirectional maturation of parallel fiber - Purkinje cell functionnally active synapses by a positive feedback mechanism. Required for CBLN3 export from the endoplasmic reticulum and secretion.
    The cerebellin peptide exerts neuromodulatory functions. Directly stimulates norepinephrine release via the adenylate cyclase/PKA-dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release.
  • Tissue specificity
    In the Purkinje cells postsynaptic structures. In the cerebellum, cerebellin is much less abundant than [des-Ser1]-cerebellin.
  • Sequence similarities
    Contains 1 C1q domain.
  • Developmental stage
    Low at birth, the cerebellin concentration increases between day 5 and 15, and reaches peak values between day 21 and 56.
  • Post-translational
    modifications
    The proteolytic processing to yield cerebellin seems to occur either prior to the secretion by presynaptic neurons and subsequent oligomerization or in some other location after release of the mature protein.
  • Cellular localization
    Secreted. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • [des-Ser1]-cerebellin antibody
    • CBLN 1 antibody
    • CBLN1 antibody
    • CBLN1_HUMAN antibody
    • CER antibody
    • Cerebellin 1 antibody
    • Cerebellin 1 precursor antibody
    • Cerebellin1 antibody
    • Precerebellin antibody
    see all

Images

  • ab64184 (10ug/ml) staining human CBLN1 in human prostate tissue by immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

References

This product has been referenced in:
  • Muguruma K  et al. Ontogeny-recapitulating generation and tissue integration of ES cell-derived Purkinje cells. Nat Neurosci 13:1171-80 (2010). Mouse . Read more (PubMed: 20835252) »

See 1 Publication for this product

Customer reviews and Q&As

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In answer to your questions:

1. Regarding ab36909 Precerebellin antibody

Based on the immunogen sequence, this antibody should detect precerebellin and cerebellin-1 (precerebellin without the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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