Overview

  • Product nameAnti-CBR1 antibody
    See all CBR1 primary antibodies
  • Description
    Goat polyclonal to CBR1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: HGQFVSEKRVEQW, corresponding to C terminal amino acids 265-277 of Human CBR1.

  • Positive control
    • Human Liver lysate.
  • General notesPrincipal Names - CBR1; CBR; carbonyl reductase 1; carbonyl reductase (NADPH); carbonyl reductase (NADPH) 1 Official Gene Symbol - CBR1 GenBank Accession Number – NP_001748 LocusLink ID - 873 (human) Gene Ontology terms - carbonyl reductase (NADPH) activity; metabolism; cytosol; oxidoreductase activity.

Properties

Applications

Our Abpromise guarantee covers the use of ab4148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 30 kDa.Can be blocked with Human CBR1 peptide (ab22991).
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

  • FunctionNADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 15 hydroxyprostaglandin dehydrogenase [NADP+] antibody
    • 15-hydroxyprostaglandin dehydrogenase [NADP+] antibody
    • Carbonyl reductase [NADPH] 1 antibody
    • Carbonyl Reductase 1 antibody
    • CBR 1 antibody
    • CBR1 antibody
    • CBR1_HUMAN antibody
    • CRN antibody
    • NADPH dependent carbonyl reductase 1 antibody
    • NADPH-dependent carbonyl reductase 1 antibody
    • Prostaglandin 9 ketoreductase antibody
    • Prostaglandin 9-ketoreductase antibody
    • Prostaglandin E(2) 9 reductase antibody
    • Prostaglandin-E(2) 9-reductase antibody
    • SDR21C1 antibody
    see all

Anti-CBR1 antibody images



  • Predicted band size : 30 kDa


    ab4148 staining (0.2µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Ab4148 (3ug/ml) staining human CBR1 in human kidney by immunohistochemistry using paraffin embedded tissue.

    Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

  • ICC/IF image of ab4148 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4148, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Lanes 1 & 10 : Protein Marker
    Lanes 2 - 9 : Anti-CBR1 antibody (ab4148) at 1/1000 dilution (in PBS + (0.05%) Tween at 25°C )

    Lane 1 : Protein Marker
    Lane 2 : Whole cell lysate of human lung cancer cell line A549 at 25 µg
    Lane 3 : Whole cell lysate of human lung cancer cell line A549 at 25 µg
    Lane 4 : Whole cell lysate of human lung cancer cell line A549 at 25 µg
    Lane 5 : Whole cell lysate of human lung cancer cell line A549 at 25 µg
    Lane 6 : 1X SDS Loading Buffer
    Lane 7 : recombinant CBR1 at 0.03 µg
    Lane 8 : recombinant CBR1 at 0.07 µg
    Lane 9 : recombinant CBR1 at 0.1 µg
    Lane 10 : Protein Marker

    Secondary
    Lanes 2 - 9 : An HRP-conjugated anti-goat IgG at 1/10000 dilution

    Developed using the ECL technique

    Predicted band size : 30 kDa
    Observed band size : 30 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

References for Anti-CBR1 antibody (ab4148)

This product has been referenced in:
  • Lim R  et al. 2D-PAGE of ovarian cancer: Analysis of soluble and insoluble fractions using medium-range immobilized pH gradients. Biochem Biophys Res Commun 406:408-13 (2011). WB ; Human . Read more (PubMed: 21329656) »
  • Catalano RD  et al. Comprehensive expression analysis of prostanoid enzymes and receptors in the human endometrium across the menstrual cycle. Mol Hum Reprod 17:182-92 (2011). IHC-P ; Human . Read more (PubMed: 21112968) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Lung cancer cell line (A549))
Loading amount 25 µg
Specification Lung cancer cell line (A549)
Gel Running Conditions Reduced Denaturing (12% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 10 2011

Thank you for your enquiry. We do not seem to have an exact match where the lysate has been extracted in RIPA buffer. My understanding is that the protein is cytoplasmic, therefore a lysate extracted in a gentler buffer should be ok. I would recom...

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Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab4148 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the e...

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Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab4148 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the e...

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Yes I would recommend your using ab6740 (Rabbit polyclonal to Goat IgG H&L (biotin)) to detect ab4148. This secondary antibody works very well in IHC - please see the two customer reviews accessible via the online datasheet. As you have seen, ab4148 ha...

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