Overview

  • Product name
    Anti-CBR1 antibody [EPR9660]
    See all CBR1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9660] to CBR1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide, corresponding to residues in Human CBR1 (Uniprot: P16152).

  • Positive control
    • HeLa, 293T, SH-SY5Y and Human fetal kidney lysates; Human kidney tissue; HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
    • Sequence similarities
      Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 15 hydroxyprostaglandin dehydrogenase [NADP+] antibody
      • 15-hydroxyprostaglandin dehydrogenase [NADP+] antibody
      • Carbonyl reductase [NADPH] 1 antibody
      • Carbonyl Reductase 1 antibody
      • CBR 1 antibody
      • CBR1 antibody
      • CBR1_HUMAN antibody
      • CRN antibody
      • NADPH dependent carbonyl reductase 1 antibody
      • NADPH-dependent carbonyl reductase 1 antibody
      • Prostaglandin 9 ketoreductase antibody
      • Prostaglandin 9-ketoreductase antibody
      • Prostaglandin E(2) 9 reductase antibody
      • Prostaglandin-E(2) 9-reductase antibody
      • SDR21C1 antibody
      see all

    Images

    • ab156590 showing +ve staining in Human thyroid gland carcinoma.
    • ab156590 showing +ve staining in Human skeletal muscle.
    • ab156590 showing +ve staining in Human gastric carcinoma.
    • All lanes : Anti-CBR1 antibody [EPR9660] (ab156590) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 30 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CBR1 with ab156590 at 1/50 dilution.
    • Immunofluorescent analysis of HeLa cells labeling CBR1 with ab156590 at 1/50 dilution.

    References

    This product has been referenced in:
    • Yamanouchi R  et al. Low carbonyl reductase 1 expression is associated with poor prognosis in patients with oral squamous cell carcinoma. Mol Clin Oncol 8:400-406 (2018). Read more (PubMed: 29456845) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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