Anti-CBS antibody [3E1] (ab124276)

Overview

  • Product nameAnti-CBS antibody [3E1]
    See all CBS primary antibodies
  • Description
    Mouse monoclonal [3E1] to CBS
  • Tested applicationsSuitable for: WB, IP, IHC-P, Sandwich ELISA, Othermore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Eecombinant fragment, corresponding to a region within N terminal amino acids 1-101 of Human CBS (NP_000062) with a 26 kDa proprietary tag.

  • Positive control
    • IHC-P: Human brain cerebellum and liver tissues WB: HeLa cell line lysate and CBS transfected 293T cell line lysate IP: CBS transfected lysate RNAi: CBS over-expressed 293 cell line lysate cotransfected with CBS Validated Chimera RNAi

Properties

Applications

Our Abpromise guarantee covers the use of ab124276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 61 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
Sandwich ELISA Use at an assay dependent concentration.
Other Use at an assay dependent concentration. RNAi Knockdown

Target

  • FunctionOnly known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.
  • Tissue specificityIn the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
  • PathwayAmino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.
  • Involvement in diseaseDefects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine.
  • Sequence similaritiesBelongs to the cysteine synthase/cystathionine beta-synthase family.
    Contains 1 CBS domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI047524 antibody
    • AI303044 antibody
    • Beta thionase antibody
    • Beta-thionase antibody
    • Cbs antibody
    • Cbs cystathionine beta-synthase antibody
    • CBS_HUMAN antibody
    • Cystathionine beta synthase antibody
    • Cystathionine beta-synthase antibody
    • EC 4.2.1.22 antibody
    • HIP 4 antibody
    • HIP4 antibody
    • Methylcysteine synthase antibody
    • MGC18856 antibody
    • MGC18895 antibody
    • MGC37300 antibody
    • OTTHUMP00000109416 antibody
    • OTTHUMP00000109418 antibody
    • Serine sulfhydrase antibody
    see all

Anti-CBS antibody [3E1] images

  • Anti-CBS antibody [3E1] (ab124276) at 1/500 dilution + HeLa cell lysate

    Predicted band size : 61 kDa
  • All lanes : Anti-CBS antibody [3E1] (ab124276) at 1/500 dilution

    Lane 1 : CBS transfected 293T cell line lysate
    Lane 2 : Non transfected 293T cell line lysate


    Predicted band size : 61 kDa
  • Immunoprecipitation of CBS transfected lysate using ab124276 and Protein A Magnetic Beads.
  • ab124276, at 5 µg/ml, staining CBS in paraffin embedded Human brain cerebellum tissue by Immunohistochemistry.
  • ab124276, at 5 µg/ml, staining CBS in paraffin embedded Human liver tissue by Immunohistochemistry.
  • Western blot analysis of RNAi Knockdown using ab124276.
    Lane 1: Non transfected control
    Lane 2: CBS over expressed 293 cell line, cotransfected with CBS Validated Chimera RNAi
    Bottom lanes: GAPDH (36.1 kDa) was used as specificity and loading control
  • ab124276, used in Sandwich ELISA against tagged recombinant Human CBS protein.
    Detection limit for ab124276 is approximately 0.1 ng/ml as a capture antibody.

References for Anti-CBS antibody [3E1] (ab124276)

ab124276 has not yet been referenced specifically in any publications.

Product Wall

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Mouse species has not been tested for antibodies ab54883 and ab124276. I am sorry we are unable to provide any feedback. If you are interested in using these antibodies with mouse species, you may consider collab...

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Both of these antibodies are absolutely fine for WB and IHC. If you want to buy one, than buy ab54883 you will get more antibody.

I hope this information is helpful to you. Please do not hesitate to co...

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There is not a huge difference between the two, mainly the concentration, product size and sELISA tested application for ab124276. Both of these antibodies are fully guaranteed to be used in applications as liste...

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