Overview

  • Product nameAnti-CBS antibody
    See all CBS primary antibodies
  • Description
    Mouse monoclonal to CBS
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MPSETPQAEV GPTGCPHRSG PHSAKGSLEK GSPEDKEAKE PLWIRPDAPS RCTWQLGRPA SESPHHHTAP AKSPKILPDI LKKIGDTPMV RINKIGKKFG , corresponding to amino acids 1-101 of Human CBS

Properties

Applications

Our Abpromise guarantee covers the use of ab54883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 60 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionOnly known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.
  • Tissue specificityIn the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
  • PathwayAmino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.
  • Involvement in diseaseDefects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine.
  • Sequence similaritiesBelongs to the cysteine synthase/cystathionine beta-synthase family.
    Contains 1 CBS domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI047524 antibody
    • AI303044 antibody
    • Beta thionase antibody
    • Beta-thionase antibody
    • Cbs antibody
    • Cbs cystathionine beta-synthase antibody
    • CBS_HUMAN antibody
    • Cystathionine beta synthase antibody
    • Cystathionine beta-synthase antibody
    • EC 4.2.1.22 antibody
    • HIP 4 antibody
    • HIP4 antibody
    • Methylcysteine synthase antibody
    • MGC18856 antibody
    • MGC18895 antibody
    • MGC37300 antibody
    • OTTHUMP00000109416 antibody
    • OTTHUMP00000109418 antibody
    • Serine sulfhydrase antibody
    see all

Anti-CBS antibody images

  • CBS antibody (ab54883) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human hepatocellular carcinoma.


  • Predicted band size : 60 kDa
    CBS antibody (ab54883) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab54883 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54883, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HepG2 cells stained with ab54883 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54883, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-CBS antibody (ab54883)

ab54883 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Mouse species has not been tested for antibodies ab54883 and ab124276. I am sorry we are unable to provide any feedback. If you are interested in using these antibodies with mouse species, you may consider collab...

Read More

Thank you for contacting us.

Both of these antibodies are absolutely fine for WB and IHC. If you want to buy one, than buy ab54883 you will get more antibody.

I hope this information is helpful to you. Please do not hesitate to co...

Read More

Thank you for contacting us.

There is not a huge difference between the two, mainly the concentration, product size and sELISA tested application for ab124276. Both of these antibodies are fully guaranteed to be used in applications as liste...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"