Overview

  • Product name
    Anti-CBS antibody [GT519]
    See all CBS primary antibodies
  • Description
    Mouse monoclonal [GT519] to CBS
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human CBS aa 39-251. The exact sequence is proprietary.
    Database link: P35520

  • Positive control
    • Raji , K562 , mouse liver , rat liver lysates
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab184219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.

Target

  • Function
    Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.
  • Tissue specificity
    In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
  • Pathway
    Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.
  • Involvement in disease
    Defects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine.
  • Sequence similarities
    Belongs to the cysteine synthase/cystathionine beta-synthase family.
    Contains 1 CBS domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI047524 antibody
    • AI303044 antibody
    • Beta thionase antibody
    • Beta-thionase antibody
    • Cbs antibody
    • Cbs cystathionine beta-synthase antibody
    • CBS_HUMAN antibody
    • Cystathionine beta synthase antibody
    • Cystathionine beta-synthase antibody
    • EC 4.2.1.22 antibody
    • HIP 4 antibody
    • HIP4 antibody
    • Methylcysteine synthase antibody
    • MGC18856 antibody
    • MGC18895 antibody
    • MGC37300 antibody
    • OTTHUMP00000109416 antibody
    • OTTHUMP00000109418 antibody
    • Serine sulfhydrase antibody
    see all

Images

  • Anti-CBS antibody [GT519] (ab184219) at 1/1000 dilution + rat liver lysate/extract at 50 µg

    Predicted band size : 61 kDa

    7.5 % SDS-PAGE

  • All lanes : Anti-CBS antibody [GT519] (ab184219) at 1/1000 dilution

    Lane 1 : Raji whole cell lysate/extract
    Lane 2 : K562 whole cell lysate/extract

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 61 kDa

    7.5 % SDS-PAGE

  • Anti-CBS antibody [GT519] (ab184219) at 1/2000 dilution + mouse liver lysate/extract at 50 µg

    Predicted band size : 61 kDa

    7.5 % SDS-PAGE

References

ab184219 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184219.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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