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Synthetic peptide, conjugated to KLH, derived from within residues 150 to the C-terminus of Human HP1 beta.
Our Abpromise guarantee covers the use of ab10478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa).|
|IP||Use at an assay dependent concentration.|
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: CBX1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: MCF7 whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab10478 observed at 26 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab10478 was shown to recognize CBX1 when CBX1 knockout samples were used, along with additional cross-reactive bands. Wild-type and CBX1 knockout samples were subjected to SDS-PAGE. Ab10478 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab10478 at 1/200 staining human cervix cells by ICC/IF. The cells were fixed in 4% PFA for 15 minutes and permeabilized for 15 minutes in 0.5% Triton X100 in PBS, prior to incubation with the antibody fort 1 hour. A Cy2 ® conjugated goat anti-rabbit antibody was used as the secondary.
Image courtesy of Human Protein Atlas
ab10478 staining CBX1 / HP1 in human testis, showing a distinct and strong staining pattern in ductus seminiferus cells. Paraffin embedded human skin tissue was incubated with ab10478 (1/400 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab10478 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org