Overview

  • Product nameAnti-CBX1 / HP1 beta antibody
    See all CBX1 / HP1 beta primary antibodies
  • Description
    Rabbit polyclonal to CBX1 / HP1 beta
  • Specificityab10478 recognises a band at 25 kDa, which is attributed to CBX1 / HP1 beta.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Marsupials
    Predicted to work with: Chicken, Hamster, Drosophila melanogaster
  • Immunogen

    Synthetic peptide, conjugated to KLH, derived from within residues 150 to the C-terminus of Human HP1 beta.

    .

  • Positive control
    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell, HeLa Nuclear, A431 Whole Cell, MCF7 Whole Cell, HEK293 Whole Cell, PC12 Whole Cell

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/100.
ICC/IF 1/200.
IP Use at an assay dependent concentration.

Target

  • FunctionComponent of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.
  • Tissue specificityExpressed in all adult and embryonic tissues.
  • Sequence similaritiesContains 2 chromo domains.
  • Post-translational
    modifications
    Not phosphorylated.
    Ubiquitinated.
  • Cellular localizationNucleus. Unassociated with chromosomes during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBX 1 antibody
    • CBX antibody
    • Cbx1 antibody
    • CBX1_HUMAN antibody
    • Chromobox 1 antibody
    • Chromobox homolog 1 (HP1 beta homolog Drosophila ) antibody
    • chromobox homolog 1 antibody
    • Chromobox protein homolog 1 antibody
    • Drosophila HP1 beta antibody
    • Heterochromatin protein 1 beta antibody
    • Heterochromatin protein 1 homolog beta antibody
    • Heterochromatin protein p25 antibody
    • heterochromatin protein p25 beta antibody
    • HP1 beta antibody
    • HP1 beta homolog antibody
    • HP1 beta homolog Drosophila antibody
    • HP1, Drosophila. homolog of, beta antibody
    • HP1beta antibody
    • HP1Hs beta antibody
    • HP1Hsbeta antibody
    • Human heterochromatin protein p25 mRNA complete cds antibody
    • M31 antibody
    • MOD 1 antibody
    • MOD1 antibody
    • Modifier 1 protein antibody
    • p25beta antibody
    see all

Anti-CBX1 / HP1 beta antibody images

  • All lanes : Anti-CBX1 / HP1 beta antibody (ab10478) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa nuclear lysate
    Lane 3 : A431 cell lysate
    Lane 4 : MCF7 cell lysate
    Lane 5 : HEK293 cell lysate
    Lane 6 : HeLa whole cell lysate with CBX1 / HP1 beta peptide (ab30861) at 1 µg/ml
    Lane 7 : HeLa nuclear lysate with CBX1 / HP1 beta peptide (ab30861) at 1 µg/ml
    Lane 8 : A431 lysate with CBX1 / HP1 beta peptide (ab30861) at 1 µg/ml
    Lane 9 : MCF7 cell lysate with CBX1 / HP1 beta peptide (ab30861) at 1 µg/ml
    Lane 10 : HEK293 cell lysate with CBX1 / HP1 beta peptide (ab30861) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Predicted band size : 21 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa (possible cross reactivity),47 kDa (possible cross reactivity).
  • CBX1 / HP1 beta was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to CBX1 / HP1 beta and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10478.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 20ka: CBX1 / HP1 beta.
  • ab10478 at 1/200 staining human cervix cells by ICC/IF. The cells were fixed in 4% PFA for 15 minutes and permeabilized for 15 minutes in 0.5% Triton X100 in PBS, prior to incubation with the antibody fort 1 hour. A Cy2 ®  conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Image courtesy of Human Protein Atlas

    ab10478 staining CBX1 / HP1 in human testis, showing a distinct and strong staining pattern in ductus seminiferus cells. Paraffin embedded human skin tissue was incubated with ab10478 (1/400 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab10478 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .
  • Anti-CBX1 / HP1 beta antibody (ab10478) at 1 µg/ml + PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • Anti-CBX1 / HP1 beta antibody (ab10478) at 1/500 dilution + Recombinant Human CBX1 / HP1 beta protein (ab109847) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Anti-CBX1 / HP1 beta antibody (ab10478)

This product has been referenced in:
  • Huang R  et al. The role of HDAC2 in chromatin remodelling and response to chemotherapy in ovarian cancer. Oncotarget 7:4695-711 (2016). WB, IHC-P ; Human . Read more (PubMed: 26683361) »
  • Thanisch K  et al. Targeting and tracing of specific DNA sequences with dTALEs in living cells. Nucleic Acids Res 42:e38 (2014). WB ; Human . Read more (PubMed: 24371265) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (smooth muscle cells)
Specification smooth muscle cells
Fixative Formaldehyde
Permeabilization Yes - 0.5% NP40/PBS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Yiwen Liu

Verified customer

Submitted Sep 25 2012

Thank you for contacting us and for your interest in our products.

I am sorry to confirm that as far as we are aware, ab10478 has never been tested with samples from zebrafish. All tested species cross-reactivity information is stated on our...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Vascular Smooth Muscle Cell)
Loading amount 20 µg
Specification Vascular Smooth Muscle Cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Andrew Cobb

Verified customer

Submitted Mar 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 50 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jan 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (Mouse Embryonic Stem Cells)
Loading amount 20 µg
Specification Mouse Embryonic Stem Cells
Gel Running Conditions Reduced Denaturing (12% Polyacrylamide)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Distal Colonic tissue Duke's Stage D Cancer)
Specification Distal Colonic tissue Duke's Stage D Cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Oct 27 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (CaCo 2 Cell line)
Loading amount 200 µg
Specification CaCo 2 Cell line
Gel Running Conditions Reduced Denaturing (15 % gel)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted Oct 27 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung carcinoma)
Specification lung carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1.5%
Username

Abcam user community

Verified customer

Submitted Sep 26 2008

Thank you for your enquiry. We update our online datasheets with new information as soon as we receive it. As you can see from the online datasheet, on February 1st we published an Abreview from a customer who successfully used the antibody in an I...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (cervix)
Specification cervix
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2%
Username

Dr. Alexander Rapp

Verified customer

Submitted Feb 01 2007

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"