Overview

  • Product nameAnti-CBX2 antibody
    See all CBX2 primary antibodies
  • Description
    Rabbit polyclonal to CBX2
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human cbx2.

    (Peptide available as ab91615.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: JEG3 whole cell; HUVEC whole cell; HEK293 whole cell; MEL-1 whole cell; MEL-2 whole cell; HeLa nuclear; NIH3T3 whole cell; human testis tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).

Target

  • FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Involved in sexual development, acting as activator of NR5A1 expression.
  • Involvement in diseaseDefects in CBX2 are the cause of 46,XY sex reversal type 5 (SRXY5) [MIM:613080]. It is a disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females.
  • Sequence similaritiesContains 1 A.T hook DNA-binding domain.
    Contains 1 chromo domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cbx 2 antibody
    • CBX2 antibody
    • CBX2_HUMAN antibody
    • CDC A6 antibody
    • CDCA6 antibody
    • Cell division cycle associated 6 antibody
    • Cell division cycle associated6 antibody
    • chromobox homolog 2 (Pc class homolog, Drosophila) antibody
    • Chromobox homolog 2 antibody
    • Chromobox homolog2 antibody
    • Chromobox protein homolog 2 antibody
    • hromobox homolog 2 antibody
    • M33 antibody
    • M33 polycomb like antibody
    • MOD 2 antibody
    • MOD2 antibody
    • Modifier 3 antibody
    • Modifier3 antibody
    • Pc class homolog antibody
    • Polycomb antibody
    • SRXY5 antibody
    see all

Anti-CBX2 antibody images

  • All lanes : Anti-CBX2 antibody (ab80044) at 1 µg/ml

    Lane 1 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
    Lane 5 : MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196)
    Lane 6 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • All lanes : Anti-CBX2 antibody (ab80044) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa


    Exposure time : 8 minutes
  • cbx2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to cbx2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab80044.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 52kDa: cbx2.

References for Anti-CBX2 antibody (ab80044)

This product has been referenced in:
  • Zhen CY  et al. Cbx2 stably associates with mitotic chromosomes via a PRC2- or PRC1-independent mechanism and is needed for recruiting PRC1 complex to mitotic chromosomes. Mol Biol Cell 25:3726-39 (2014). WB ; Mouse . Read more (PubMed: 25232004) »
  • Klauke K  et al. Polycomb Cbx family members mediate the balance between haematopoietic stem cell self-renewal and differentiation. Nat Cell Biol 15:353-62 (2013). WB . Read more (PubMed: 23502315) »

See all 2 Publications for this product

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Thank you for contacting us.

In reviewing your previous emails I see that you wish to use ab80044 in IP on mouse. We have already tested this product in IP and on mouse as such this product is covered by our Abpromise guarantee. Should thi...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
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Dr. Kirk McManus

Verified customer

Submitted Apr 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"