Recombinant
RabMAb

Anti-CBX2 antibody [EPR12624] - C-terminal (ab184968)

Overview

  • Product name
    Anti-CBX2 antibody [EPR12624] - C-terminal
    See all CBX2 primary antibodies
  • Description
    Rabbit monoclonal [EPR12624] to CBX2 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CBX2 aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14781

  • Positive control
    • HeLa, HEK293 and NIH:OVCAR-3 cell lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR12624
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Flow Cyt 1/220.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Involved in sexual development, acting as activator of NR5A1 expression.
  • Involvement in disease
    Defects in CBX2 are the cause of 46,XY sex reversal type 5 (SRXY5) [MIM:613080]. It is a disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females.
  • Sequence similarities
    Contains 1 A.T hook DNA-binding domain.
    Contains 1 chromo domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cbx 2 antibody
    • CBX2 antibody
    • CBX2_HUMAN antibody
    • CDC A6 antibody
    • CDCA6 antibody
    • Cell division cycle associated 6 antibody
    • Cell division cycle associated6 antibody
    • chromobox homolog 2 (Pc class homolog, Drosophila) antibody
    • Chromobox homolog 2 antibody
    • Chromobox homolog2 antibody
    • Chromobox protein homolog 2 antibody
    • hromobox homolog 2 antibody
    • M33 antibody
    • M33 polycomb like antibody
    • MOD 2 antibody
    • MOD2 antibody
    • Modifier 3 antibody
    • Modifier3 antibody
    • Pc class homolog antibody
    • Polycomb antibody
    • SRXY5 antibody
    see all

Images

  • All lanes : Anti-CBX2 antibody [EPR12624] - C-terminal (ab184968) at 1/50000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : NIH:OVCAR-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

  • Flow cytometric analysis of CBX2 expression in paraformaldehyde-fixed HeLa cells using ab184968 at 1/220 dilution (red) and a rabbit IgG as negative control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

References

ab184968 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue (mouse embryonic gonad sections)
Permeabilization
No
Specification
mouse embryonic gonad sections
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
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Submitted Jun 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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