Overview

  • Product nameAnti-cbx7 antibody
    See all cbx7 primary antibodies
  • Description
    Rabbit polyclonal to cbx7
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Chimpanzee
  • Immunogen

    Synthetic peptide derived from within residues 150 - 250 of Human cbx7.

    (Peptide available as ab22423.)

  • Positive control
    • This antibody gave a positive signal in MEF1 whole cell lysate and HeLa nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab31765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

Target

  • RelevanceControl of cell proliferation by Polycomb group proteins (PcG) is an important facet of cellular homeostasis and its disruption can promote tumorigenesis. CBX7 is a novel PcG protein which is found to control the growth of normal cells. CBX7 is a gene that encodes a Polycomb protein, as shown by sequence homology, its interaction with Ring1 and its localization to nuclear Polycomb bodies. CBX7 extends the lifespan of a wide range of normal human cells and immortalizes mouse fibroblasts by downregulating expression of the Ink4a/Arf locus. CBX7 does not inter-function or colocalize with Bmi1, and both can exert their actions independently of each other as shown by reverse genetics. CBX7 was highly expressed in three different prostate cancer cell lines and present at elevated levels in normal prostate. (Gil et al., 2004).
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Cbx 7 antibody
    • CBX7 antibody
    • Chromobox homolog 7 antibody
    • Chromobox protein homolog 7 antibody

Anti-cbx7 antibody images

  • All lanes : Anti-cbx7 antibody (ab31765) at 1 µg/ml

    Lane 1 : Hela nuclear Lysate
    Lane 2 : Hela nuclear Lysate with Human cbx7 peptide (ab22423) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Secondary ab: Alexa Fluor Goat polyclonal to Rabbit IgG (700)

    Predicted band size : 28 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-cbx7 antibody (ab31765) at 1 µg/ml + MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-cbx7 antibody (ab31765)

This product has been referenced in:
  • Maertens GN  et al. Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation of the INK4a tumour suppressor. EMBO J 29:2553-65 (2010). WB ; Human . Read more (PubMed: 20601937) »
  • Gelsomina_Mansueto, Floriana_Forzati, Angelo_Ferra Identification of a New Pathway for Tumor Progression Genes & Cancer 1:210 (2010). Read more (PubMed: ) »

See all 2 Publications for this product

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