• Product name
  • Description
    Rabbit polyclonal to cbx7
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 201-251 (DADLAEGPPP WTPALPSSEV TVTDITANSI TVTFREAQAA EGFFRDRSGK F), of Human cbx7 (NP_783640.1).

  • Positive control
    • HeLa or 293T cell lysate



Our Abpromise guarantee covers the use of ab91431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 28 kDa.
IP Use at 5-15 µg/mg of lysate.


  • Relevance
    Control of cell proliferation by Polycomb group proteins (PcG) is an important facet of cellular homeostasis and its disruption can promote tumorigenesis. CBX7 is a novel PcG protein which is found to control the growth of normal cells. CBX7 is a gene that encodes a Polycomb protein, as shown by sequence homology, its interaction with Ring1 and its localization to nuclear Polycomb bodies. CBX7 extends the lifespan of a wide range of normal human cells and immortalizes mouse fibroblasts by downregulating expression of the Ink4a/Arf locus. CBX7 does not inter-function or colocalize with Bmi1, and both can exert their actions independently of each other as shown by reverse genetics. CBX7 was highly expressed in three different prostate cancer cell lines and present at elevated levels in normal prostate. (Gil et al., 2004).
  • Cellular localization
  • Database links
  • Alternative names
    • Cbx 7 antibody
    • CBX7 antibody
    • Chromobox homolog 7 antibody
    • Chromobox protein homolog 7 antibody

Anti-cbx7 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling cbx7 with ab91431 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-cbx7 antibody (ab91431) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 28 kDa

    Exposure time : 10 seconds
  • cbx7 was immunoprecipitated from HeLa whole cell lysate, using ab91431 at 10 µg/mg of lysate. 20% of the immunoprecipitae was loaded per lane for the subsequent Western blot and probed with ab91431 at 1 µg/ml (lane 1) or a control IgG (lane 2).
    Detetion: chemoluminescence with exposure time of 30 seconds.

References for Anti-cbx7 antibody (ab91431)

ab91431 has not yet been referenced specifically in any publications.

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