Overview

  • Product name
  • Description
    Rabbit polyclonal to Cbx8
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residue 350 and the C-terminus (residue 389) of human Cbx8 (NP_065700.1).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab70796 was affinity purified using an epitope specific to Cbx8 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
    • Sequence similarities
      Contains 1 chromo domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cbx8 antibody
      • CBX8_HUMAN antibody
      • Chromobox Homolog 8 (Drosophila Pc Class) antibody
      • Chromobox Homolog 8 (Pc Class Homolog, Drosophila) antibody
      • Chromobox Homolog 8 antibody
      • Chromobox protein homolog 8 antibody
      • hPc3 antibody
      • Pc Class 3 Homolog (Drosophila) antibody
      • Pc Class 3 Homolog antibody
      • Pc3 antibody
      • Polycomb 3 antibody
      • Polycomb 3 homolog antibody
      • RC1 antibody
      • Rectachrome 1 antibody
      see all

    Anti-Cbx8 antibody images

    • All lanes : Anti-Cbx8 antibody (ab70796) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 43 kDa
      Observed band size : 43 kDa
      Additional bands at : 28 kDa,55 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Detection of Cbx8 by Western Blot of Immunoprecipitate.
      ab70796 at 1µg/ml staining Cbx8 in HeLa whole cell lysate immunoprecipitated using ab70796 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 minutes.

    References for Anti-Cbx8 antibody (ab70796)

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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