Overview

  • Product name
  • Description
    Goat polyclonal to Cbx8
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide with sequence C-RERGTSRVDDKPSS, from the internal region of the human protein Cbx8 (NP_065700.1)

  • Positive control
    • K562 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab77361 is purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/32000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 43 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
    • Sequence similarities
      Contains 1 chromo domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cbx8 antibody
      • CBX8_HUMAN antibody
      • Chromobox Homolog 8 (Drosophila Pc Class) antibody
      • Chromobox Homolog 8 (Pc Class Homolog, Drosophila) antibody
      • Chromobox Homolog 8 antibody
      • Chromobox protein homolog 8 antibody
      • hPc3 antibody
      • Pc Class 3 Homolog (Drosophila) antibody
      • Pc Class 3 Homolog antibody
      • Pc3 antibody
      • Polycomb 3 antibody
      • Polycomb 3 homolog antibody
      • RC1 antibody
      • Rectachrome 1 antibody
      see all

    Images

    • Anti-Cbx8 antibody (ab77361) at 0.1 µg/ml + K562 cell lysate at 35 µg

      Predicted band size : 43 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

    References

    ab77361 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77361.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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