The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 104 kDa).
Use at 3-10 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
CC2D1A belongs to the CC2D1 family. It contains one C2 domain. It is a transcription factor that binds specifically to the FRE (five repressor element) and represses HTR1A gene transcription in neuronal cells. The combination of calcium and ATP specifically inactivates the binding with FRE. CC2D1A may play a role in the altered regulation of HTR1A associated with anxiety and major depression. It also mediates HDAC-independent repression of HTR1A promoter in neuronal cell. There are three named isoforms. Defects in CC2D1A are the cause of non-syndromic mental retardation autosomal recessive type 3 (MRT3). Patients display severe mental retardation and psychomotor development delay in early childhood. Non-syndromic mental retardation patients do not manifest other clinical signs.
Detection of Human CC2D1A by Immunoprecipitiaton. ab70375 used at 20 µg/50 mg NETN or RIPA lysate from HeLa cells. Immunoprecipitate was resolved by SDS-PAGE and the band that is CC2D1A was confirmed by mass spectrometry.