• Product nameAnti-CCDC11 antibody
    See all CCDC11 primary antibodies
  • Description
    Rabbit polyclonal to CCDC11
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (YSQRFGTVQR EVKGPTPKVV IVRSKPPKGQ GAEHHLERIR RSHQKHNAIL) of Human CCDC11 (NP_659457).

  • Positive control
    • 293T cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab84324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).Can be blocked with CCDC11 peptide (ab117880). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


Anti-CCDC11 antibody images

  • Anti-CCDC11 antibody (ab84324) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
  • ab84324 staining CCDC11 in human skin fibroblasts by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformladehyde, permeabilized using 0.25% Triton X-100/PBS, blocked with 5% serum for 30 minutes at 25°C and then incubated with ab84324 at 1µg/ml for 3 hours at 25°C. The secondary used was an FITC conjugated donkey anti-rabbit IgG (H+L) used at a 1/500 dilution. Nuclear staining with DAPI.

    See Abreview

References for Anti-CCDC11 antibody (ab84324)

This product has been referenced in:
  • Perles Z  et al. A human laterality disorder associated with recessive CCDC11 mutation. J Med Genet 49:386-90 (2012). ICC/IF ; Human . Read more (PubMed: 22577226) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Skin Fibroblasts)
Specification Skin Fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.25% Triton X-100 in PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 28 2011

Thank you for your message which has been forwarded to me as my colleague Miia is currently away from the office. We do request that customers submit their Abreview before the expirey date in order to make use of this offer. However, we understand ...

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Thank you for contacting us. The blocking peptide for the Anti-CCDC11 antibody (ab84324) is ab117880. This was previously not in our catalogue but has now been added due to your request. This may not be possible in every case but if any of your...

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